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C3867

Sigma-Aldrich

Rat Collagen Type I

Collagen, Type I solution from rat tail

from rat tail, liquid, 4 mg/mL, suitable for cell culture

Synonym(s):

Collagen from rat tail, Col1a1

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About This Item

CAS Number:
9007-34-5
EC Number:
232-697-4
MDL number:
MFCD00130825
UNSPSC Code:
12352202
NACRES:
NA.75
Pricing and availability is not currently available.

Product Name

Collagen, Type I solution from rat tail, BioReagent, suitable for cell culture, sterile-filtered

biological source

rat tail

Quality Level

200

description

Approx. 100mg/vial ((35 - 40 ml) )

sterility

sterile-filtered

product line

BioReagent

Assay

>95% (SDS-PAGE)

form

liquid

mol wt

apparent mol wt 115-130 kDa by SDS-PAGE (doublet)
apparent mol wt 215-235 kDa by SDS-PAGE (doublet)

packaging

pkg of 1 vial

concentration

>0.5 mg/mL Biuret

technique(s)

cell culture | mammalian: suitable

surface coverage

6‑10 μg/cm2

impurities

Mycoplasma Test by Barile, none detected

solubility

soluble, clear to hazy, colorless (solubilized in 0.02M acetic acid)

NCBI accession no.

NM_053304.1

UniProt accession no.

P02454

Binding Specificity

Peptide Source: Collagen

Peptide Source: Fibronectin

shipped in

wet ice

storage temp.

2-8°C

Gene Information

rat ... Col1a1(29393)

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General description

Collagen is an extracellular matrix protein comprising triple helical structure. The structure of collagen type I, in particular, is characterized by the presence of three identical α-chains in its homotrimetric form. Among these three α-chains, one exhibits slight sequence variation.[1] Type I collagen was first isolated from rat-tail tendon, has two main α chains (α and α ) and one β chain.[2]

Application

Collagen, Type I solution from rat tail has been used for coating culture plates and dishes for human hepatocyte HepG2 cell line[3] and podocytes.[4] It has also been used as a standard for total collagen content calibration curve generation for the quantification of liver extracellular matrix pre-gel.[5]
Used as a coating material to support adherent cells growth and differentiation.

Biochem/physiol Actions

Collagen protein is essential for the mechanical integrity of tendons and bone. Rat tail tendon collagen is used in tissue engineering especially in the generation of 3-D scaffolds based gels.[6] It has low antigenicity and is compatible with human gingival fibroblasts and human oral keratinocytes.[2]

Features and Benefits

  • Sourced from rat tail.
  • Sterile-filtered to minimize contamination risks.
  • Suitable for a wide range of cell culture applications.
  • Provides optimal surface coverage (6-10 μg/cm2) for promoting cell adhesion and proliferation.

Preparation Note

Recommended for use as a cell culture substratum. May not be suitable for 3-D gel formation.
Sterile solution prepared from rat tail tendons by a modification of the published extraction method 1. It is supplied as an aqueous solution in 20mM acetic acid with a protein concentration as stated on the bottle. (approx.100mg protein per vial). Protein concentration was estimated by the Biuret method 2.
SDS polyacrylamide gel electrophoresis analysis shows the typical band pattern for Type I collagen, with a doublet at apparent molecular weights of 115 and 130kDa and another doublet at 215 and 235kDa. Based on this analysis, the purity of the collagen sample is >95%.

Other Notes

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.
When C3867 is first removed from the cooler it will appear gelled. When it is allowed to come fully to 37°C it will appear as a liquid.

related product

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000407189
0000397140
0000391989
0000386369
0000385522

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Type I collagen extracted from rat-tail and bovine Achilles tendon for dental application: a comparative study
Techatanawat S, et al.
Asian Biomedicine : Research, Reviews and News, 5(6), 787-798 (2011)
Thermal helix-coil transition in UV irradiated collagen from rat tail tendon
Sionkowska A and Kaminska A
International Journal of Biological Macromolecules, 24(4), 337-340 (1999)
Preparation of ready-to-use, storable and reconstituted type I collagen from rat tail tendon for tissue engineering applications
Rajan N, et al.
Nature Protocols, 1(6), 2753-2753 (2006)
On the adhesion-cohesion balance and oxygen consumption characteristics of liver organoids
Mattei G, et al.
Testing, 12(3), e0173206-e0173206 (2017)
Mechanisms of angiotensin II signaling on cytoskeleton of podocytes
Hsu HH, et al.
Journal of Molecular Medicine, 86(12), 1379-1394 (2008)
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Questions

1–9 of 9 Questions  

  1. Should I add collagen I to acetic acid to obtain 0.1% (w/v) collagen solution, or it's already added and I should only dilute it to working concentration which is 0.01?

    1 answer

    1. Here are our preparation instructions:
      This product is prepared from rat tail tendons by modification of a published procedure.
      It is supplied as an aqueous solution in 20 mM acetic acid (~100 mg of protein per vial). Protein concentration was estimated by the biuret method.
      The vial contains approximately 100 mg of protein. Volume per vial: 22–25 ml.  The product ships on wet ice and storage at 2–8 °C is recommended. During shipment, or if the storage temperature for this product goes slightly below the recommended range, the product may acquire a gel-like appearance. Under such circumstances, it is possible to warm the product gently, to no more than 45 °C, in order to reconstitute the product fully, before use. Aliquots that have been diluted to a working concentration may be stored at 2–8 °C for up to 2 weeks. Do not freeze.  Optimal conditions for attachment must be determined for each cell line and application.

      1. An appropriate volume of the collagen solution should be diluted to a working concentration of 0.01% using sterile, tissue culture grade water.
      2. Coat dishes with 6-10 mg/cm2. Allow the protein to bind for several hours at room temperature or 37 °C, or overnight at 2–8 °C.
      3. Remove excess fluid from the coated surface and allow it to dry overnight. If sterility has been compromised, the dried, coated surface can be sterilized easily by overnight exposure to UV light in a sterile tissue culture hood.
      4. Rinse with sterile tissue culture grade water or a balanced salt solution before introducing cells and medium.

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  2. What will be the concentration of collagen? I am trying to download the CoA, but I am unable to download it.

    1 answer

    1. This product is prepared at a concentration of approximately 4 mg/mL. A package of 1 Vial will typically have a volume of approximately 35-40 mL. The exact concentration is lot specific and reported in the product Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
      https://www.sigmaaldrich.com/product/sigma/c3867#product-documentation

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  3. IVD conformity: Does this product have IVDD or IVDR conformity?

    1 answer

    1. This collagen is intended for research use only, and is not approved for IVD purposes.

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  4. In what volume should I dilute this solution in order to make collagen-coated plates for tissue culture?

    1 answer

    1. The recommended surface coverage for this product is 6?10 μg/cm2. Collagen solutions may be diluted to a working concentration of 0.01%. A general collagen coating protocol is available at https://www.sigmaaldrich.com/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/mammalian-cell-culture/collagen-product-protocols

      The collagen concentration of this product is reported on a lot specific basis and will be required to prepare the working solution. See the link below to review a sample Certificate of analysis:
      https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/C3867-1VL0000255665.pdf

      Helpful?

  5. How does the storage temperature relate to shipping conditions?

    1 answer

    1. The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.

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  6. How are collagen solutions stored?

    1 answer

    1. Collagen solutions should be stored in the refrigerator at 2-8 °C for up to 1 year, unless it becomes contaminated.

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  7. How can I make a 3-D collagen gel?

    1 answer

    1. Not all lots of C7661 are suitable for 3D gels. Product No. C4243 has been use-tested in this application. If C4243 is not available, we recommend that customers screen lots of C7661 to try to make 3D gels. We have found that collagen that has been sterilized by gamma-irradiation or by chloroform cannot be used to make 3D collagen gels. The following protocol can be used to make collagen gels: Materials required:Collagen gel solution 1.5 - 3 mg/mL 10X tissue culture medium containing phenol red Sodium bicarbonate or HEPES buffer Procedure: 1.Measure out 800 μL of collagen solution. 2.Add 100 μL of 10X medium (buffered with 1X sodium bicarbonate or HEPES) 3.Adjust pH with 1 N sodium hydroxide, if required (100 μl or less) 4.Add 10X medium to bring volume to 1 ml 5.Mix contents well. Solution should maintain red color to indicate physiological pH6. Dispense into wells to a depth of 1-2 mm (approx. 15 μL/well) 7.Transfer to 37 °C for 20-40 minutes 8.Examine for gel formation The above volumes represent quantities for use in 24 well plates. Volumes can be adjusted to accommodate any culture vessel.

      Helpful?

  8. Why am I seeing some insolubles in my collagen solution?

    1 answer

    1. The solubility specifications for this product is clear to hazy colorless solution with a few insolubles at 1 mg/ml in water with 2 μl acetic acid (or 0.1 N acetic acid). The insolubles can be removed by settling or centrifugation.

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  9. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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