B2551

Brewer thioglycollate medium

suitable for microbiology, NutriSelect^® Plus

• Synonym(s): Thioglycollate Medium (Brewer)
• UNSPSC Code: 41171607
• NACRES: NA.85

Properties

• sterility: non-sterile
• Quality Level: 200
• form: powder
• shelf life: limited shelf life, expiry date on the label
• manufacturer/tradename: NutriSelect^® Plus
• pH: 7.2±0.2 (25 °C)
• application(s): clinical testing; food and beverages; sterility testing; microbiology
• suitability: anaerobic bacteria; nonselective for Bacteroides spp.; nonselective for Clostridium spp.; nonselective for Streptococcus spp.; nonselective for bacteria (General Media)

Description

Application

Used for testing the sterility of biological products.

Components

Components (g/L)
Proteose Peptone 10.00
Infusion from Beef 17.50
Dextrose 5.00
Sodium Chloride 5.00
Dipotassium Phosphate 2.00
Sodium Thioglycollate 0.50
Methylene Blue 0.002
Agar 0.50

Preparation Note

Suspend 40.5 g of Brewer Thioglycollate Medium in 1000 ml of distilled water. Boil to dissolve the medium completely. Dispense in tubes or in suitable containers as desired and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes.Note: If more than the upper one third of medium has acquired a green color, the medium may be restored once by heating on a water bath or free flowing steam until the green color disappears.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H317
• Precautionary Statements: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves