11373242910

Fluorescein-12-dUTP

≥85% (HPLC), suitable for ELISA, suitable for hybridization, solution

• Synonym(s): Fluorescein-12-dUTP, dUTP
• UNSPSC Code: 41116100

Properties

• Quality Level: 100
• Assay: ≥85% (HPLC)
• form: solution
• mol wt: M_r 1018.4 (fluorescein-12-dUTP-Li₄)
• packaging: pkg of 25 μL (25 nmol; 1mM)
• manufacturer/tradename: Roche
• technique(s): ELISA: suitable; hybridization: suitable
• storage temp.: −20°C

Description

Application

Fluorescein-12-dUTP is used as a substrate for:

• terminal transferase
• DNA polymerase I (holoenzyme and Klenow fragment),
• Taq DNA polymerase
• reverse transcriptase (e.g., from AMV and M-MuLV).

Fluorescein-12-dUTP has been used to label nuclear extracts during PCR.[1] It has also been used to replace dTTP in the random-primed DNA labeling reaction or in nick translation reactions, as well as in PCR. The nucleotide also serves as a substrate for terminal transferase in 3′-end labeling and TUNEL.[2] Fluorescein-labeled probes can be used for in in situ hybridization with direct fluorescence detection[3] or detection by enzyme linked immunoimmunsorbent assay (ELISA) using Anti-Fluorescein-AP, Fab fragments. Repeated fluorescence labeling using Tetramethyl-Rhodamine-6-dUTP (red) and AMCA-6-dUTP (bright blue) is possible.

Quality

Typical analysis: At least 85% Fluorescein-12-dUTP (HPLC, area%).

Physical form

Fluorescein-12-dUTP, tetralithium salt.

Storage and Stability

The unopened reagent is stable at 15 to 25°C until the expiration date printed on the label. Decomposition of approx. 5% may occur within 6 months.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): does not flash
• Flash Point(C): does not flash