QIA127
Rapid Cell Proliferation Kit
Synonym(s):
Proliferation assay kit
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.84
Recommended Products
usage
sufficient for 500 tests
Quality Level
packaging
pkg of 1 96-well plate(s)
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
protect from light
input
sample type cultured cells (1,000-50,000 cells)
detection method
colorimetric
shipped in
wet ice
storage temp.
−20°C
General description
Assay measures the increased activity of cellular mitochondrial dehydrogenases that can cleave the tetrazolium dye WST-1 to formazan. The formazan formation is then quantified by measuring the change in absorbance at 450 nm in a microplate reader. The activity of mitochondrial dehydrogenases is proportional to cell number. No washing, harvesting, or solubilization steps are required.
Features and Benefits
Sensitivity: 1000 cells (adherent); 2500 cells (nonadherent)
Components
WST-1, 1-Methoxy PMS, and a user protocol.
Warning
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Specifications
Assay Time: 0.5 - 4 h
Principle
The Calbiochem Rapid Cell Proliferation Kit is designed to determine the number of viable cells in proliferation, or cytotoxicity studies, based on the cleavage of the tetrazolium salt WST-1 by mitochondrial dehydrogenases in viable cells. The assay can be used to measure cell proliferation in response to growth factors or cytokines, or to assess cytotoxic compounds.
Preparation Note
• WST-1 Labeling Mixture: Thaw the EMS solution at room temperature or in a 37°C water bath. Add the entire contents of this solution to the bottle containing the WST-1 reagent. The mixed solution can be stored at 4°C for several weeks. For long-term storage (several months) aliquot and store at -20°C. Protect from light.
Other Notes
Ishiyama, M., et al. 1995. In Vitro Toxicology8, 187.
Liu, S.O., et al 1995. Nat. Med.1, 267.
Ishiyama, M., et al. 1993. Chem. Pharm. Bull.41, 1118.
Liu, S.O., et al 1995. Nat. Med.1, 267.
Ishiyama, M., et al. 1993. Chem. Pharm. Bull.41, 1118.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
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