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PAT001

Sigma-Aldrich

Protein A-Turbo - Proximity Biotinylation Enzyme

Synonym(s):

ProteinA-Turbo ID

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.46

form

liquid

Quality Level

200

purified by

affinity chromatography

packaging

pkg of 100 μg

technique(s)

affinity binding assay: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
mass spectrometry (MS): suitable
proximity ligation assay: suitable

storage temp.

-65 to -96°C

General description

Protein A Turbo is a recombinantly expressed Protein A-TurboID fusion protein with the capability to bind IgG through the Protein A region, and enzymatically biotinylate proximal proteins non-specifically with the TurboID region.

Specificity

Protein A strongly binds multiple Immunoglobulin G subclasses (IgG1, IgG2, IgG4), but does not bind IgG3. This probe is not compatible with primary antibodies that are IgG3.

Application

  • Proximity protein labeling
  • Cell Biology
  • Histology
  • Fixed cell microscopy
  • Tissue staining
  • Immunofluorescence
  • Liquid Chromatography
  • Mass Spectrometry
  • Immunoprecipitation


Physical form

Purified protein in 20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, 150 mM imidazole with protease inhibitor cocktail.

Reconstitution

0.7 mg/mL

Storage and Stability

Store at -65°C to -96°C.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H315,H319,H360D

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Storage Class Code

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Irene Santos-Barriopedro et al.
Nature communications, 12(1), 5015-5015 (2021-08-20)
Proximity biotinylation workflows typically require CRISPR-based genetic manipulation of target cells. To overcome this bottleneck, we fused the TurboID proximity biotinylation enzyme to Protein A. Upon target cell permeabilization, the ProtA-Turbo enzyme can be targeted to proteins or post-translational modifications

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