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MABC199

Sigma-Aldrich

Anti-BRCA1 Antibody, clone MS110

clone MS110, from mouse

Synonym(s):

Breast cancer type 1 susceptibility protein, RING finger protein 53

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

MS110, monoclonal

species reactivity

human

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... BRCA1(672)

General description

Breast cancer type 1 susceptibility protein (BCRA1; RING finger protein 53) functions as a tumor suppressor. BRCA1 is an important member of the DNA repair pathway. After DNA damage, BRCA1 is phosphorylated on multiple serine residues by the ATR kinase or Chk2, and is localized to lesion sites with PCNA. BRCA1 interacts with a wide range of proteins including Rad50, NBS1, and Mre11, c-Abl tyrosine kinase, and γH2A.X, involved in the detection of damaged DNA and activation of appropriate repair pathways. BRCA1 may also respond to DNA damage by promoting gene expression through association with transcriptional proteins and RNA polymerase II, and may regulate the p53 pathway by this mechanism. Previous studies have indicated that mutations in the BRCA1 gene may contribute to the development of breast and ovarian cancer.

Specificity

This antibody recognizes the N-terminus of BRCA1.

Immunogen

Epitope: N-terminus
Recombinant protein corresponding to the N-terminus of human BRCA1.

Application

Anti-BRCA1 Antibody, clone MS110 is an antibody against BRCA1 for use in Western Blotting, ICC.
Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected in BRCA1 in MCF7 cells.

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated BRCA1 in IP (Wilson, C. A., et al. (1999). Nat Genet. 21(2):236-240.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected BRCA in ovarian cancer and breast cancer tissues (Scully, R., et al. (1996). Science. 272(5258):123-126; Wilson, C. A., et al. (1999). Nat Genet. 21(2):236-240.).

Immunofluorescence Analysis: A representative lot from an independent laboratory detected BRCA1 in a variety of certain cell lines (Scully, R., et al. (1996). Science. 272(5258):123-126.).
Research Category
Apoptosis & Cancer
Research Sub Category
Cell Cycle, DNA Replication & Repair

Quality

Evaluated by Western Blot in HeLa nuclear extract.

Western Blot Analysis: 1 µg/mL of this antibody detected BRCA1 in 10 µg of HeLa nuclear extract.

Target description

~220 kDa and ~85 kDa observed. Multiple isoforms exist at various molecular weights and may be subject to post-translational modification.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa nuclear extract

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Giulia Partipilo et al.
International journal of cancer, 138(1), 110-120 (2015-07-25)
Understanding the expression of proteins involved in DNA damage response could improve knowledge of the pathways that contribute to familial and sporadic breast cancer (BC). We aimed to assess the different roles of BRCA1, poly(ADP-ribose) polymerase-1 (PARP1), BRCT-repeat inhibitor of
Yunlong Qin et al.
American journal of cancer research, 2(5), 540-548 (2012-09-08)
Mutation in the BRCA1 gene is associated with increased risk for hereditary breast and ovarian cancers. In sporadic ovarian tumors, BRCA1 dysfunction is thought to be common. BRCA1 is a nuclear-cytoplasm shuttling protein. Our group has previously reported that BRCA1
Brian P Schlegel et al.
Cancer research, 66(10), 5181-5189 (2006-05-19)
The BRCA1 tumor suppressor contributes to the repair of DNA double-strand breaks (DSB) through homologous recombination, but the mechanism is unknown. The rapid accumulation of BRCA1 into nuclear foci in response to induction of DNA breaks suggests that BRCA1 may
Aaron Seo et al.
Proceedings of the National Academy of Sciences of the United States of America, 115(20), 5241-5246 (2018-05-02)
BRCA1 is essential for repair of DNA double-strand breaks by homologous recombination, and hence for survival. Complete loss of its function is lethal during early embryonic development. Patients who are compound heterozygous for BRCA1 truncating mutations and missense alleles that
Khwanjira Hongthong et al.
Heliyon, 7(8), e07749-e07749 (2021-08-26)
RAPTA-EA1 is a promising glutathione transferase (GSTP-1) inhibitor that has previously been shown to inhibit the growth of various breast cancer cells. We studied the anticancer activity of RAPTA-EA1 on triple-negative BRCA1 competent breast cancer MDA-MB-231 cells. MDA-MB-231 cells are

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