IL029
Interleukin-12 Protein, Recombinant human
The recombinant human Interleukin-12 protein (or IL-12 protein) is a potent regulator of cell mediated immune responses & it induces IFN-gamma production by NK & T cells.
Synonym(s):
IL-12
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About This Item
UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.77
Recommended Products
biological source
human
Quality Level
recombinant
expressed in CHO cells
Assay
>98% (SDS-PAGE and HPLC)
specific activity
≥1.0 x 10E6 U/mg
manufacturer/tradename
Chemicon®
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 ng/μg endotoxin (of IL-12; 1EU/μg)
input
sample type hematopoietic stem cell(s)
shipped in
dry ice
General description
Interleukin-12 (IL-12) is a potent regulator of cell mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes, and connective tissue type mast cells. Among its biological activities, IL-12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells and induces the development of IFN-gamma producing Th1 cells. Human IL-12 is a disulfide linked heterodimeric protein with a molecular weight of 75 kDa comprised of disulfide-bonded 35 kDa (p35) and 40 kDa (p40) subunits.
Product Source: CHO cells (Chinese Hamster Ovarian cells)
Application
For most in vitro applications, IL-12 exerts its biological activity in the concentration range of 1.0 to 10.0 ng/mL.
Biochem/physiol Actions
The ED50 as determined by the induction of IFN-g production from NK cells co-stimulated with IL-18 was ≤ 1.0 ng/mL
Physical form
The sterile filtered solution was lyophilized from 0.5X PBS pH 7.2
Storage and Stability
The lyophilized IL-12, though stable at room temperature, is best stored desiccated below 0°C. Reconstitute with sterile water to a concentration of 0.2 mg/mL. Reconstituted IL-12 should be stored in undiluted aliquots at -20°C for up to 6 months.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Certificates of Analysis (COA)
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Jiyun Kim et al.
Biomicrofluidics, 9(4), 044109-044109 (2015-09-05)
Fabrication methods for the development of multiplexed immunoassay platforms primarily depend on the individual functionalization of reaction chambers to achieve a heterogeneous reacting substrate composition, which increases the overall manufacturing time and cost. Here, we describe a new type of
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