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AB4070

Sigma-Aldrich

Anti-DAP12 Antibody

Chemicon®, from rabbit

Synonym(s):

Anti-DAP12, Anti-KARAP, Anti-PLOSL, Anti-PLOSL1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, human

manufacturer/tradename

Chemicon®

technique(s)

immunoprecipitation (IP): suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... TYROBP(7305)

Related Categories

General description

DAP12 is a transmembrane adaptor protein that is non-covalently associated with several cell surface receptors on natural killer (NK), myeloid, and presumably neuronal cells (Bakker, 2000, Campbell, 1999). DAP12 contains an ITAM domain that preferentially recruits the protein tyrosine kinase Syk to initiate signal transduction (McVicar, 1998). Deficiency of DAP12 results in reduced antigen presentation function by myeloid cells and defects in function of certain NK cell receptors in mice (Bakker. 2000, Tomasello, 2000), as well as presenile dementia and bone cysts in humans (Paloneva, 2000).

Specificity

Recognizes DAP12 protein.

Immunogen

Recombinant DAP12 protein.

Application

Anti-DAP12 Antibody detects level of DAP12 & has been published & validated for use in IP.
Immunoprecipitation (2-4 μg/mg of protein lysate)

Optimal working dilutions must be determined by end user.
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology

Physical form

Format: Purified
Purified immunoglobulin. Liquid in phosphate buffered saline with 0.08% sodium azide.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
POSITIVE CONTROL: NKp44 positive cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Vanessa Montalvo et al.
Immunology, 140(4), 475-482 (2013-08-03)
DNAX-activation protein 12 (DAP12), a transmembrane adapter, plays a major role in transducing activation signals in natural killer cells and various myeloid cells. Quantitative RT-PCR detected in normal mouse eyes considerable levels of DAP12 and multiple DAP12-coupled receptors, in particular
Mariela Chertoff et al.
PloS one, 8(8), e72083-e72083 (2013-08-27)
During postnatal development, microglia, the resident innate immune cells of the central nervous system are constantly monitoring the brain parenchyma, cleaning the cell debris, the synaptic contacts overproduced and also maintaining the brain homeostasis. In this context, the postnatal microglia
Masaaki Kobayashi et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 36(43), 11138-11150 (2016-11-01)
Neuropathic pain afflicts millions of people, and the development of an effective treatment for this intractable pain is an urgent issue. Recent evidence has implicated microglia in neuropathic pain. The present study showed that the DNAX-activating protein of 12 kDa

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