539131

Protease Inhibitor Cocktail I

lyophilized, for the inhibition of aspartic, cysteine, serine, metalloproteases and aminopeptidases

• Synonym(s): Protease Inhibitor Mix, Protease inhibitor
• UNSPSC Code: 12352200
• NACRES: NA.28

Properties

• product name: Protease Inhibitor Cocktail Set I, A cocktail of five protease inhibitors that will inhibit a broad range of proteases and esterases. Supplied with a data sheet.
• form: lyophilized
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic)
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Protease Inhibitor Cocktail Set I A is specially formulated containing five protease inhibitors that will inhibit a broad range of proteases and esterases.

Specificity

Inhibits a broad range of proteases and esterases.

Application

Protease Inhibitor Cocktail Set I – Calbiochem has been used in lysis buffer to lyse cells and perform western blotting.

Protease Inhibitor Cocktail Set I have been used as a supplement in:

• cold radioimmunoprecipitation assay (RIPA) buffer to prepare cell lysates for western blotting
• RIPA lysis buffer to extract total protein from rat liver tissues and AML12 cells
• sucrose solution to homogenize mice cortices for myelin isolation

Biochem/physiol Actions

Protease inhibitor cocktails and specific protease inhibitors are essential tools utilized by scientists in various proteomic studies. These studies encompass protein expression, characterization, biomarker discovery, mapping post-translational modifications, protein expression profiling, and quantitative protein measurement. The application of a protease inhibitor cocktail or protease inhibitors guarantees the preservation of protein integrity, facilitating downstream analysis and characterization.

Features and Benefits

• Protease Inhibitor Cocktails offer protection of target protein against degradation in crude extracts
• Ready for reconstitution

Warning

Toxicity: Harmful (C)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.

Reconstitute each vial with 1 ml dH2O to yield a 100X stock solution.

Other Notes

The presence of EDTA may interfere with the purification of His•Tag fusion proteins if a cell extract is applied directly to IMAC resins.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS08,GHS05,GHS07
• Signal Word: Danger
• Hazard Statements: H314,H332,H373
• Precautionary Statements: P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P314
• Hazard Classifications: Acute Tox. 4 Inhalation - Eye Dam. 1 - Skin Corr. 1A - STOT RE 2 Inhalation
• Target Organs: Respiratory Tract
• Storage Class Code: 8B - Non-combustible corrosive hazardous materials
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable