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48-680MAG

Millipore

MILLIPLEX MAP Multi-Pathway Magnetic Bead 9-Plex - Cell Signaling Multiplex Assay

Synonym(s):

ERK Cell Signaling Assay, Luminex® Cell Signaling Assay, Millipore Cell Signaling Assay

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.47
Pricing and availability is not currently available.

species reactivity

human

Quality Level

200

manufacturer/tradename

Milliplex®

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

storage temp.

2-8°C

Related Categories

General description

The Milliplex® MAP 9-plex Multi-Pathway Signaling Magnetic Bead kit, phosphoprotein, is used to detect changes in phosphorylated ERK/MAP kinase 1/2 (Thr185/Tyr187), Akt (Ser473), STAT3 (Ser727), JNK (Thr183/Tyr185), p70S6K (Thr412), NFkB (Ser536), STAT5A/B (Tyr694/699), CREB (Ser133), and p38 (Thr180/Tyr182) in cell lysates using the Luminex® xMAP® technology. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures. Each kit has sufficient reagents for one 96-well plate assay.

Specificity

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Application

Analyze a cell signaling panel using this kit. The analytes available for the cell signaling multiplex assay kit are: CREB (pS133), ERK (pT185/pY187), NFκB (pS536), JNK (pT183/pY185), p38 (Thr180/Tyr182), p70S6K (Thr412), STAT3 (pS727), STAT5A/B (pY694/699), Akt (pS473).
Research Category
Cell Signaling
Research Sub Category
Oncology
Used to detect/quantify:CREB (pS133)|ERK (pT185/pY187)|NFκB (pS536)|JNK (pT183/pY185)|p38 (Thr180/Tyr182)|p70S6K (Thr412)|STAT3 (pS727)|STAT5A/B (pY694/699)|Akt (pS473)

Storage and Stability

Recommended storage for kit components is 2 - 8°C

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

Signal Word

Danger

Hazard Statements

H302,H315,H318,H410

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Questions

1–3 of 3 Questions  

  1. Can you explain what the Amplification Buffer is in Cell Signaling Panels?

    1 answer

    1. The use of the Amplification Buffer is proprietary. Its primary purpose is to enhance the signal of phosphorylated proteins.

      Helpful?

  2. Is there a protocol available for running the 96-well Milliplex kits in a 384-well format?

    1 answer

    1. We do not provide a recommended protocol for 384-well conversion. However, the Custom Assay Team (CADI) offers this as a service.

      Helpful?

  3. Are the Milliplex Cell Signaling panels qualitative? If so, what settings are used in xPonent for analysis? Can the data be normalized in Belysa?

    1 answer

    1. The Cell Signaling kits are qualitative, and the xPonent Analysis method is not applicable. Belysa does not offer a qualitative option.

      Helpful?

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