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208748

Sigma-Aldrich

Calpain-1 Substrate, Fluorogenic

An internally quenched fluorogenic substrate peptide derived from the calpain-1 cleavage site of α-spectrin.

Synonym(s):

Calpain-1 Substrate, Fluorogenic, μ-Calpain Substrate, Fluorogenic, H-K(FAM)-EVY~GMMK(DABCYL)-OH

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About This Item

Empirical Formula (Hill Notation):
C79H95N13O19S2
Molecular Weight:
1594.81
UNSPSC Code:
12352200
NACRES:
NA.77

Quality Level

Assay

≥95% (HPLC)

form

lyophilized solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated (hygroscopic)
protect from light

color

red

solubility

DMSO: 1 mg/mL

fluorescence

λex ~490 nm
λem ~518 nm

shipped in

wet ice

storage temp.

−20°C

General description

An internally quenched fluorogenic substrate peptide derived from the calpain-1 cleavage site of α-spectrin. It is not recognized by trypsin or α-chymotrypsin and serves as a sensitive and specific substrate for calpain-1 (Km = 4.6 µM; kcat = 11 s-1). Cleavage occurs at the Tyr-Gly bond and results in enhanced fluorescence. Excitation max: ~490 nm; emission max: ~518 nm.
An internally quenched fluorogenic substrate peptide derived from the calpain-1 cleavage site of α-spectrin. It is not recognized by trypsin or α-chymotrypsin and serves as a sensitive and specific substrate for calpain-1 (Km = 4.6 µM; kcat = 11 s-1). Cleavage occurs between Tyr-Gly residues and results in enhanced fluorescence.

Biochem/physiol Actions

Cell permeable: no
Primary Target
A sensitive and specific substrate for calpain-1
Product does not compete with ATP.
Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Sequence

H-Lys(FAM)-Glu-Val-Tyr~Gly-Met-Met-Lys(DABCYL)-OH

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Mittoo, S., et al. 2003. Anal. Biochem.319, 234.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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David S Dang et al.
Meat science, 162, 108039-108039 (2020-01-15)
The purpose of this study was to examine the role of mitochondria in postmortem calcium homeostasis and its effect on proteolysis and tenderness. We hypothesized that mitochondria buffer cytosolic calcium levels and delay the activation of calpain-1 and subsequently the

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