16-157
Protein A Agarose/Salmon Sperm DNA, 2.5 mL
for use in chromatin immunoprecipitations (ChIP assays)
Synonym(s):
ChIP agarose beads, ChIP agaraose A beads, ChIP assays
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.32
Recommended Products
biological source
Staphylococcus aureus
Quality Level
form
liquid
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable
suitability
suitable for immunoprecipitation
shipped in
wet ice
General description
Protein A covalently bound to agarose by alkylamine linkage containing sonicated Salmon Sperm DNA. The salmon sperm DNA blocks non-specific interactions with the beads for use in chromatin IP (ChIP) assays. Note part number 16-157C present in the ChIP kits is not available separately, please purchase 16-157.
Protein A from Staphylococcus aureus has a very high affinity for the Fc regions of IgG molecules. Immoblisation of protein A on beads creates an affinity resin which can be used to purify IgG fractions from crude serum, ascites fluid or cell cuture media.
Application
Suitable for use in chromatin immunoprecipitation (ChIPs) assays
Quality
Routinely evaluated in a Chromatin Immunoprecipitation by pulling down DNA cross-linked to acetylated histones; subsequent detection was performed using anti-Acetyl Histone H4, ChIPs Grade (06-866)
Physical form
10mM Tris-HCl, 1mM EDTA, pH 8.0 containing 0.05% sodium azide.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Mohammad Kamran et al.
Journal of immunology (Baltimore, Md. : 1950), 205(12), 3311-3318 (2020-11-15)
IL-13 plays a critical role in mediating many biological processes responsible for allergic inflammation. Mast cells express Il13 mRNA and produce IL-13 protein in response to antigenic stimulation. Enhancers are essential in promoting gene transcription and are thought to activate
Generation of p53 target database via integration of microarray and global p53 DNA-binding site analysis.
Suxing Liu, Asra Mirza, Luquan Wang
Methods in Molecular Biology null
A note on penicillin resistance and beta-lactamase production in strains of Staphylococcus aureus recovered from different clinical materials in Nsukka, Nigeria.
Oguike, J U, et al.
The Journal of Communicable Diseases, 23, 200-201 (1991)
Requirement of Smad3 and CREB-1 in mediating transforming growth factor-beta (TGF beta) induction of TGF beta 3 secretion.
Guangming Liu, Wei Ding, Jill Neiman, Kathleen M Mulder
The Journal of Biological Chemistry null
In vitro analysis of the anthelmintic activity of forage chicory (Cichorium intybus L.) sesquiterpene lactones against a predominantly Haemonchus contortus egg population.
Foster JG, Cassida KA, Turner KE
Veterinary Parasitology null
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service