813125

TSKgel^® Boronate-5PW (20 µm) HPLC Columns

L x I.D. 2.5 cm × 6 mm, m-aminophenyl boronate phase

• Sinónimos: TSKgel^® Boronate-5PW HPLC Guardgel Kit
• UNSPSC Code: 41115711
• eCl@ss: 32110501

Propiedades

• product name: TSKgel^® Boronate-5PW HPLC Guardgel Kit, phase m-aminophenyl boronate, L × I.D. 2.5 cm × 6 mm, 20 μm particle size
• material: PEEK
• product line: TSKgel^®
• manufacturer/tradename: Tosoh Boronate-5PW, 13125
• parameter: ≤20% organic solvent; ≤3 M salt concentration; ≥ 0 °C temperature
• technique(s): HPLC: suitable
• L × I.D.: 2.5 cm × 6 mm
• matrix: polymer particle platform
• matrix active group: m-aminophenyl boronate phase
• particle size: 20 μm
• pore size: 1000 Å
• operating pH: 2-9
• capacity: 40 μmol/mL, resin adsorption capacity (sorbitol)
• compatibility: mode of use affinity HPLC
• separation technique: affinity
• storage temp.: 2-8°C

Descripción

General description

Guardgel Kit for 7.5mmID TSKgel Boronate-5PW columns

High efficiency, resin-based TSKgel affinity columns (10 μm particles) separate or purify many enzymes and other proteins.

Coupling of m-aminophenyl boronate to TSKgel 5PW polymeric support results in a ligand capable of forming a tetrahedral boronate anion under alkaline pH conditions. This anionic structure can bind with 1,2 cis-diol groups such as those found in carbohydrates, carbohydrate containing compounds and catecholamines. Interaction between the boronate anion and the 1,2 cis-diol groups is enhanced in the presence of Mg2+ ions and is inhibited by amine-containing buffers. Adsorption onto the TSKgel Boronate-5PW matrix takes place in basic buffers such as HEPES and morpholine while desorption takes place in carbohydrate or amine-containing mobile phase like sorbitol or Tris.

Applications include: nucleic acids, catecholamines, nucleosides and nucleotides.

Components

TSKgel Guardgel kits contain:

• 5 mL Guardgel packing material
• Column holder, consisting of external reducing union, cap, frit, 2 ferrules
• Column, 25 mm x 9.5 mm OD x 6 mm ID, 2 μm frit at 10 mm depth, 1/16"
• 2 Replacement frits
• Capillary tubing, 5 cm x 1/16" OD x 0.01"
• Nuts and ferrules, 10-32 threads for 1/16" tubing, 2 each
• All hardware is 316 stainless steel

Dimensions for Guardgel kits for 21.5 mm ID columns:

• Column, 35 mm x 12.75 mm OD x 10 mm ID, 10 μm frit at 20mm depth, 1/16"
• Capillary tubing, 5 cm x 1/16" OD x 0.02" ID

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Legal Information

TSKgel is a registered trademark of Tosoh Corporation