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ZMS1046

Sigma-Aldrich

Anti-Rabies glycoprotein Antibody, clone 24-3F-10 ZooMAb® Mouse Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.43

origen biológico

mouse

Nivel de calidad

recombinante

expressed in HEK 293 cells

conjugado

unconjugated

forma del anticuerpo

purified antibody

tipo de anticuerpo

primary antibodies

clon

24-3F-10, recombinant monoclonal

Línea del producto

ZooMAb® learn more

Formulario

lyophilized

mol peso

calculated mol wt 58.66 kDa

purificado por

using protein G

reactividad de especies

virus

envase

antibody small pack of 25 μL

características de los productos alternativos más sostenibles

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

validación mejorada

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

técnicas

flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable

isotipo

IgG2a

secuencia del epítopo

Unknown

Nº de acceso Protein ID

Nº de acceso UniProt

categoría alternativa más sostenible

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

modificación del objetivo postraduccional

unmodified

Descripción general

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Especificidad

Clone 24-3F-10 is a ZooMAb® Mouse recombinant monoclonal antibody that detects glycoprotein of Rabies virus.

Inmunógeno

Protein G of Evelyn Rokitniki Abelseth(ERA) strain of Rabies virus.

Aplicación

Quality Control Testing

Evaluated by Immunocytochemistry in L929 cells infected with Rabies virus.

Immunocytochemistry Analysis (ICC): A 1:100 dilution of this antibody detected Rabies virus glycoprotein in L929 cells infected with Rabies virus.

Tested applications

Immunofluorescence Analysis: A representative lot detected Rabies glycoprotein in Immunofluorescence applications (Do-Phong, Johnny., et. al. (2016). Elife. 5:e13214).

Flow Cytometry Analysis: 0.1 μg from a representative lot detected Rabies glycoprotein in one million L929 cells infected with Rabies virus.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Rabies glycoprotein, clone 24-3F-10 ZooMAb®, Cat. No. ZMS1046, is a recombinant Mouse monoclonal antibody that detects Rabies virus glycoprotein and is tested for use in Flow Cytometry, Immunocytochemistry, and Immunofluorescence.

Descripción de destino

Glycoprotein (UniProt: P03524) is encoded by the G gene in Rabies virus, strain ERA. Glycoprotein is a single-pass type I membrane glycoprotein that belongs to the lyssavirus glycoprotein family. It attaches the virus to host cellular receptor, inducing endocytosis of the virion. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and cell membrane. The muscle form of the nicotinic acetylcholine receptor (nAChR), the neuronal cell adhesion molecule (NCAM), and the p75 neurotrophin receptor (p75NTR) are reported to bind glycoprotein and thereby facilitate rabies virus entry into cells. Glycoprotein is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off in the mature form. Its structure includes a virion surface (aa 20-459), helical region (aa 460-480) and an intravirion (aa 481-524). Arginine 352 is reported to be involved in Rabies virus pathogenicity and its mutation attenuates the virus. It is glycosylated and palmitoylated by host and its glycosylation is considered to be crucial for its export at the cell surface. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Forma física

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb® formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Almacenamiento y estabilidad

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Información legal

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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