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Merck

T3195

Sigma-Aldrich

Anti-γ-Tubulin Antibody

rabbit polyclonal

Sinónimos:

Anti-CDCBM4, Anti-GCP-1, Anti-TUBG, Anti-TUBGCP1

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.43

Nombre del producto

Anti-γ-Tubulin (DQ-19) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution

origen biológico

rabbit

Nivel de calidad

conjugado

unconjugated

forma del anticuerpo

IgG fraction of antiserum

tipo de anticuerpo

primary antibodies

clon

polyclonal

Formulario

buffered aqueous solution

mol peso

antigen 48 kDa

reactividad de especies

chicken, human

técnicas

indirect immunofluorescence: 1:500 using methanol-acetone fixed, cultured chicken fibroblasts.
western blot: 1:1,000 using whole cell extract of human epidermal carcinoma A431 cell line.

Nº de acceso UniProt

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

Descripción general

Tubulin (48 kDa) is a widely expressed and highly conserved protein within the microtubule organizing centers (MTOCs) or centrosome in eukaryotic cells. It is a member of the tubulin superfamily of proteins which includes α- and β- tubulin and the newly discovered centrosomal-associated proteins, δ- and ε- tubulin. γ-Tubulin is a part of large protein complex containing at least five other proteins and has a shape of a ring (γ-tubulin ring complex, γ-TuRC) that is roughly the same diameter as a microtubule. Its abundance is less than 1% of the level of either α- or β-tubulin. γ-Tubulin shares 28-32% identity with α-tubulin from various organisms, 32-36% identity with β-tubulins and 29-30% identity with δ- and ε tubulins. Some regions including those thought to be involved in GTP) binding are highly conserved among α-, β-, γ-, δ-, and ε-tubulins.

Inmunógeno

synthetic peptide corresponding to the C-terminus of human γ-tubulin (amino acids 433-451 with N-terminally added lysine), conjugated to KLH.

Aplicación

Anti-γ-Tubulin (DQ-19) antibody produced in rabbit has been used in:
  • western blotting
  • immunocytochemistry
  • immunofluorescence

Acciones bioquímicas o fisiológicas

The proper organization of microtubules is essential for cell division and chromosome segregation, directed cell movement, interphase cytoplasmic organization, and other cytoskeletal functions. γ-Tubulin has an essential role in microtubule nucleation by the centrosomes. It binds to the β-tubulin half of the tubulin molecule, thus establishing the polarity of a microtubule, leaving the α-tubulin half exposed at the plus end. γ-TuRC (γ-tubulin ring complex) binds the microtubule minus ends and is responsible for mediating the link between microtubules and the centrosome.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Nihal Kaplan et al.
The ocular surface, 21, 193-205 (2021-06-14)
To understand the relationship between ciliogenesis and autophagy in the corneal epithelium. siRNAs for EphA2 or PLD1 were used to inhibit protein expression in vitro. Morpholino-anti-EphA2 was used to knockdown EphA2 in Xenopus skin. An EphA2 knockout mouse was used
The centrosomal kinase Aurora-A/STK15 interacts with a putative tumor suppressor NM23-H1
Du J and Hannon GJ
Nucleic Acids Research, 30(24), 5465-5475 (2002)
Microtubule-organizing centres: a re-evaluation
Luders J and Stearns T
Nature Reviews in Molecular and Cell Biology, 8(2), 161-161 (2007)
The elongation of primary cilia via the acetylation of a-tubulin by the treatment with lithium chloride in human fibroblast KD cells.
Nakakura T
Medical Molecular Morphology, 48(1), 44-53 (2015)
Mohan Singh et al.
Experimental hematology, 44(12), 1181-1187 (2016-09-11)
Primary cilia are nonmotile, microtubule-based organelles that are present on the cellular membrane of all eukaryotic cells. Functional cilia are required for the response to developmental signaling pathways such as Hedgehog (Hh) and Wnt/β-catenin. Although the Hh pathway has been

Artículos

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.

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