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Merck

SRP3085

Sigma-Aldrich

Interleukin-2, human

Animal-component free, recombinant, expressed in E. coli, suitable for cell culture

Sinónimos:

Aldesleukin, IL-2, T-cell growth factor (TCGF)

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

human

Quality Level

recombinant

expressed in E. coli

assay

≥95% (HPLC)

form

(Lyophilized with no additives from a 0.2µ filtered solution)

potency

≤0.5 ng/mL ED50

mol wt

15.5 kDa

packaging

pkg of 50 μg

technique(s)

cell culture | mammalian: suitable

impurities

≤1.00 EU/μg endotoxin, tested

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... IL2(3558)

General description

IL-2 (interleukin 2) is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is a type I cytokine. The gene is mapped to human chromosome 4q27. Recombinant human IL-2 is a 15.5kDa protein, containing 134 amino acid residues including one intrachain disulfide bond.

Application

  • IL-2 (interleukin 2) human has been used for the detection of IL-2 using 4-Fluoro-3-nitrophenyl grafted gold electrode based platform.
  • It has been used for the production of tumor-specific cytotoxic T lymphocytes.
  • It has been used for the proliferation of T lymphocytes.

Biochem/physiol Actions

IL-2/IL-2R (interleukin 2/IL-2 receptor) signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK (natural killer) cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes. It is associated with recurrent oral ulcer and asthma and Mycoplasma pneumoniae infection in children. Absence of IL2 in mice causes fatal lymphoproliferative inflammatory disease (IL-2 deficiency syndrome). IL-2 signaling involves three pathways: phosphoinositol 3-kinase (PI3-K)/AKT, Ras-MAPK (mitogen activated protein kinase), and JAK-STAT (Janus kinase - signal transducers and activators of transcription) pathways.

Sequence

MAPTSSSTKK TQLQLEHLLL DLQMILNGIN NYKNPKLTRM LTFKFYMPKK ATELKHLQCL EEELKPLEEV LNLAQSKNFH LRPRDLISNI NVIVLELKGS ETTFMCEYAD ETATIVEFLN RWITFAQSII STLT

Physical form

Lyophilized with no additives.

Reconstitution

Reconstitute the contents of the vial using 100 mM acetic acid to a concentration of 0.1-1.0 mg/mL. This solution can then be diluted into other aqueous buffers and stored at 2-8 °C for up to 1 week. For extended storage, freeze in working aliquots. It may be advisable to centrifuge the vial prior to reconstitution.

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1


Certificados de análisis (COA)

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Visite la Librería de documentos

A mechanism of hypoxia-mediated escape from adaptive immunity in cancer cells.
Barsoum IB
Cancer Research, 74, 665-674 (2014)
The common gamma-chain cytokines IL-2, IL-7, IL-15, and IL-21 induce the expression of programmed death-1 and its ligands.
Kinter AL
Journal of Immunology, 181, 6738-6746 (2008)
4-Fluoro-3-nitrophenyl grafted gold electrode based platform for label free electrochemical detection of interleukin-2 protein.
Arya SK and Park MK
Biosensors And Bioelectronics, 61, 260-265 (2014)
Prostaglandin E2 (PGE2)-dependent suppression of interleukin alpha (IL-2) production in patients with major trauma.
Faist E
The Journal of Trauma, 27, 837-848 (1987)
Family based association analysis of the IL2 and IL15 genes in allergic disorders.
Christensen U
European Journal of Human Genetics, 14, 227-235 (2006)

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