S7313
S-Gal® sodium salt
reagent for selection of recombinant bacterial clones
Sinónimos:
3,4-Cyclohexeneoesculetin-B-D-galactopyranoside sodium salt
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
Fórmula empírica (notación de Hill):
C19H21O9Na
Peso molecular:
416.35
Número MDL:
Código UNSPSC:
12352200
NACRES:
NA.85
Productos recomendados
grado
for molecular biology
esterilidad
non-sterile
Ensayo
≥95% (HPLC)
Formulario
powder
DIV
not for in vitro diagnostic use
solubilidad
H2O: 100 mg/mL
idoneidad
suitable for β-galactosidase test
temp. de almacenamiento
room temp
Descripción general
S-Gal® (sodium salt) is an autoclavable, water-soluble, chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. S-gal® is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
Aplicación
S-Gal, sodium salt is a patented water-soluble, autoclavable chromogenic substrate for β-galactosidase that is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
Características y beneficios
- More intense color contrast than X-gal
- Water-soluble and autoclavable for easiest use
- Excellent for use in automated colony counters
- No need to make stock solutions
When S-Gal is cleaved by β-galactosidase, the resulting product will chelate ferric ion to create a black, insoluble precipitate. Lac+ colonies grown in the presence of S-Gal and ferric ion turn an intense black color, allowing for easy differentiation between lac+ and lac- colonies. S-Gal, Sodium salt is water soluble, eliminating the need for solvents such as dimethyl formamide. S-Gal is also autoclavable and can be added to your medium of choice prior to autoclaving. S-Gal is not light sensitive and does not require any protection from light sources.
Otras notas
More water soluble version, soluble >50 mg/mL.
The ferric or Fe3+ ion is required for color development and must be added to any S-Gal®
formulation. A medium prepared with S-Gal® is moderately dark due to the presence of ferric ammonium citrate. This darker background often provides enhanced contrast for automated colony counting or isolation.
formulation. A medium prepared with S-Gal® is moderately dark due to the presence of ferric ammonium citrate. This darker background often provides enhanced contrast for automated colony counting or isolation.
Precaución
For black color development to occur, ferric ion must be present. Although we recommend ferric ammonium citrate (500 mg/L of media), other ferric compounds can be used to provide this requirement, depending upon your particular system.
Principio
When S-Gal® is cleaved by ß-galactosidase, the resulting product will chelate ferric ion to create a black, insoluble precipitate. Lac+ colonies grown in the presence of S-Gal® and ferric ion turn an intense black color, allowing for easy differentiation between lac+ and lac- colonies.
Ligadura / enlace
Ferric Ammonium Citrate (F5879), LB Agar (L2897), IPTG (Isopropyl β-D-thiogalactopyranoside, I6758), Ampicillin (A2804), Kanamycin (K0879), Chloramphenicol (C7795), Tetracycline (T8032)
Reconstitución
Add to agar media pre-autoclaving at a recommended concentration of 300 mg S-Gal/L of media, along with 500 mg/L Ferric Ammonium Citrate (F5879). Stock solutions of S-Gal can be made by dissolving at 50 mg/mL in deionized water, sterile-filtering and storing at -20 °C.
Stock solutions of S-Gal® (sodium salt) can be made by dissolving 50mg/ml in water, dimethyl formamide (DMF) or DMSO. Filter sterilize and store at -20C. Add S-gal® (300 mg/L from stock solution or powder) and Ferric Ammonium Citrate (500mg/L) to agar media prior to autoclaving.
Información legal
S-GAL is a registered trademark of Merck KGaA, Darmstadt, Germany
Producto relacionado
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
Elija entre una de las versiones más recientes:
Certificados de análisis (COA)
Lot/Batch Number
¿No ve la versión correcta?
Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Los clientes también vieron
Winfried Hense et al.
PLoS biology, 5(10), e273-e273 (2007-10-12)
Genes with male- and testis-enriched expression are under-represented on the Drosophila melanogaster X chromosome. There is also an excess of retrotransposed genes, many of which are expressed in testis, that have "escaped" the X chromosome and moved to the autosomes.
Weina Cui et al.
Magnetic resonance in medicine, 64(1), 65-71 (2010-06-24)
Reporter genes and associated enzyme activity are becoming increasingly significant for research in vivo. The lacZ gene and beta-galactosidase (beta-gal) expression have long been exploited as reporters of biologic manipulation at the molecular level, and a noninvasive detection strategy based
S-Gal?, A novel 1H MRI reporter for ?-galactosidase?
Weina Cui
Magnesium Research (2010)
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico