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Merck

S6022

Sigma-Aldrich

Sephadex® G-50

BioReagent, for molecular biology, DNA grade, medium

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About This Item

Número de CAS:
Número MDL:
Código UNSPSC:
23151817
NACRES:
NA.52

grado

DNA grade
for molecular biology

Nivel de calidad

Línea del producto

BioReagent

Formulario

beads

hinchazón

1 g swells to 9-11 mL

diámetro microesfera

50-150 μm

tamaño de poro

exclusion limit (20 bp dsDNA; (rA)20)

pH

2-10

actividad extraña

DNase, RNase, none detected

temp. de almacenamiento

room temp

cadena SMILES

O1C(C(C(C(C1CO)O)O)O)OCC2OC(C(C(C2O)O)O)OCC(O)C(O)C(O)C(O)C=O

InChI

1S/C18H32O16/c19-1-5(21)9(23)10(24)6(22)3-31-17-16(30)14(28)12(26)8(34-17)4-32-18-15(29)13(27)11(25)7(2-20)33-18/h1,5-18,20-30H,2-4H2

Clave InChI

FZWBNHMXJMCXLU-UHFFFAOYSA-N

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Descripción general

Sephadex® G-50, Medium is an often-cited gel filtration medium for desalting and buffer exchange of biomolecules > 30,000 MW. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Medium” indicates a large particle size for use in large-scale group separations where high flow rates and low operating pressures are required.

Aplicación

Sephadex® G-50 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify PCR products prior to DNA sequencing reactions.
Sephadex® G-50 has been used to purify the DNA products in cycle sequencing reactions.

Características y beneficios

  • Excellent recovery
  • Minimal sample dilution
  • Quick and easy to use

Información legal

Sephadex is a registered trademark of Cytiva

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)


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