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P3563

Sigma-Aldrich

Phosphate Buffered Saline

powder, pH 7.4, with 0.05% Tween® 20

Sinónimos:

1X PBS + 0,05 % TWEEN® 20, PBS-T

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About This Item

Código UNSPSC:
41105319
eCl@ss:
32129211
NACRES:
NA.25
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Nombre del producto

Disolución salina tamponada con fostato al 0,05 % TWEEN® 20, pH 7,4,

Formulario

powder

Nivel de calidad

200

solubilidad

water: soluble, clear, colorless (1 pouch dissolved in 1L)

aplicaciones

cell analysis
diagnostic assay manufacturing

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Descripción general

La disolución salina tamponada con fosfato con Tween®-20, o PBST, se utiliza principalmente en inmunoanálisis como un tampón de lavado y de bloqueo. El tampón fosfato mantiene un pH 7,4 estable, adecuado para muchas reacciones y análisis biológicos. La sal o disolución salina en PBS-T proporciona la fuerza iónica necesaria para una solubilidad y estabilidad proteicas óptimas. La adición de un 0,05% de TWEEN®-20, un detergente no iónico, contribuye a reducir las señales de fondo al bloquear los sitios de unión inespecíficos de las proteínas. El tampón PBST mejora la eficiencia de lavado al eliminar sustancias no deseadas y limita los sitios de unión inespecífica de los anticuerpos, al tiempo que preserva la integridad de las proteínas diana.

Aplicación

La disolución salina tamponada con fosfato se ha utilizado en:

  • inmunoelectrotransferencia (western blot)
  • enzimoinmunoanálisis de absorción (ELISA)[1]
  • telómero/FISH (Tel-FISH) y telómero/inmunotinción-FISH (Teli-Fish)[2][3][4]
  • aislamiento celular[5]

Envase

Bolsas de aluminio

Reconstitución

Contenido de una bolsa disuelto en 1 l de agua dH2O o agua ultrapura para producir disolución salina tamponada con fosfato 0,01M,NaCl 0,138M, KCl 0.0027M y 0,05% de Tween 20, pH7,4.

Otras notas

Si desea más información sobre nuestra gama de productos bioquímicos, cumplimente este formulario.

Información legal

TWEEN is a registered trademark of Croda International PLC

Código de clase de almacenamiento

13 - Non Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000393719
0000391849
0000342977
0000379944
0000371889

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PLoS neglected tropical diseases, 12(3), e0006312-e0006312 (2018-03-02)
Mass drug administration (MDA) of ivermectin has become the main intervention to control onchocerciasis or "river blindness". In Togo, after many years of MDA, Onchocerca volvulus infection has declined dramatically, and elimination appears achievable, but in certain river basins the
Telomere length assessment in human archival tissues: combined telomere fluorescence in situ hybridization and immunostaining
Meeker A K, et al.
The American Journal of Pathology, 160(4), 1259-1268 (2002)
Expression of the galectin-9-Tim-3 pathway in glioma tissues is associated with the clinical manifestations of glioma
Liu Z, et al.
Oncology Letters, 11(3), 1829-1834 (2016)
The alternative lengthening of telomeres phenotype in breast carcinoma is associated with HER-2 overexpression
Subhawong A P, et al.
Modern Pathology, 22(11), 1423-1423 (2009)
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Protocolos

ELISA Procedures

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

ELISA Procedures

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

ELISA Procedures

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

ELISA Procedures

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

Questions

1–3 of 3 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. I am looking for the expiry date of P3563, lot number SLCP6390 please and thanks. It is not printed on the COA received with the item.

    1 answer

    1. This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.

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