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NA0200

Sigma-Aldrich

GenElute™ HP Plasmid Midiprep Kit

greener alternative

sufficient for 25 purifications

Sinónimos:

GenElute™ HP Plasmid Midiprep Kit, HP midiprep kit, fast midiprep kit, fast plasmid isolation kit, plasmid extraction kit, plasmid isolation kit, plasmid purification kit, ultrafast plasmid isolation kit, Gen Elute

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About This Item

Código UNSPSC:
41105501
NACRES:
NA.52
En este momento no podemos mostrarle ni los precios ni la disponibilidad

uso

sufficient for 25 purifications

Nivel de calidad

200

características de los productos alternativos más sostenibles

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

técnicas

DNA purification: suitable

categoría alternativa más sostenible

, Aligned

temp. de almacenamiento

15-25°C

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Descripción general

The GenElute HP Plasmid Midiprep Purification kits offer an ultrafast and efficient solution for large-scale plasmid preparation from E. coli cultures. These kits combine silica-based membrane technology and the convenience of spin or vacuum format. The kits also include a special filter cartridge, which replaces the centrifugation step following alkaline lysis. Following lysis the DNA is bound to the silica membrane and contaminants are removed with a simple wash step. The DNA is then eluted in water or TE buffer by centrifugation.

The purified plasmid DNA is suitable for a wide variety of molecular biology applications, including restriction digestions and sequencing.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Aplicación

GenElute™ HP Plasmid Midiprep Kit has been used to isolate and purify plasmids.[1][2]

Características y beneficios

  • From harvested bacterial culture to pure plasmid DNA in 30 minutes or less
  • Up to 350 μg of high-copy plasmid DNA
  • Flexibility of a vacuum or spin format
  • No phenol/chloroform extraction or alcohol precipitation required
  • Contains fewer plastic components, reducing the amount of waste

Otras notas

For additional information, please see www.sigma-aldrich.com/genelutehp.

Información legal

GenElute is a trademark of Sigma-Aldrich Co. LLC

Palabra de señalización

Danger

Clasificaciones de peligro

Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3

Órganos de actuación

Central nervous system

Código de clase de almacenamiento

3 - Flammable liquids

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Certificados de análisis (COA)

Lot/Batch Number
0000385570
0000333635
0000385570
0000333635
SLCP2413

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Despite the importance of Aβ aggregation in Alzheimer's disease etiology, our understanding of the sequence determinants of aggregation is sparse and largely derived from in vitro studies. For example, in vitro proline and alanine scanning mutagenesis of Aβ40 proposed core
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Gays F
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Questions

1–6 of 6 Questions  

  1. How long does it take to isolate plasmid using the vacuum format?

    1 answer

    1. A midiprep or maxiprep requires only 30 minutes to complete.

      Helpful?

  2. Do the binding columns fit on other manufacturers’ manifolds?

    1 answer

    1. Yes. The columns fit on all standard vacuum manifolds.

      Helpful?

  3. How do the GenElute HP Plasmid Kits differ from other plasmid purification systems?

    1 answer

    1. The GenElute HP Plasmid Kits are much faster than other commercially available kits. A filter syringe is used for preparing the cleared lysate, which replaces a lengthy centrifugation step. These kits also offer the option of using a vacuum or spin purification format rather than a gravity drip to bind, wash and elute the plasmid. In addition, samples are eluted in Tris buffer so there are no alcohol precipitations required.

      Helpful?

  4. Which format gives a better yield, vacuum or spin?

    1 answer

    1. There is no difference in plasmid recovery when using either format.

      Helpful?

  5. What is the expected yield of plasmid DNA that you can isolate from these kits?

    1 answer

    1. Yields can reach as high as 350 mg for a midiprep and 1.2 mg for a maxiprep. As with any protocol, lower yields can be expected with low copy plasmids.

      Helpful?

  6. Is plasmid DNA isolated with this kit suitable for use in transfection?

    1 answer

    1. Yes. However, if you are currently required to use an endofree plasmid purification kit, we recommend using the GenElute HP Endotoxin-free Kit.

      Helpful?

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