The key distinction lies in Product No. MP0030, LookOut® Mycoplasma Elimination Kit, which actively eradicates mycoplasma by disrupting the membrane. As a lipopeptide, it identifies cholesterol-rich regions in the membrane (as the concentration of cholesterol in mycoplasma membranes is higher than in eukaryotic cells), creating a pore through which the surrounding medium flows into the mycoplasma, ultimately causing it to burst. In contrast, standard antibiotics simply inhibit metabolic pathways, necessitating the rinsing out of mycoplasma through a medium change. If the process is halted prematurely, any remaining mycoplasma can regrow.
MP0030
LookOut® Mycoplasma Elimination Kit
Sinónimos:
Mycoplasma contamination treatment
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.75
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usage
kit sufficient for 5 mL mycoplasma elimination
Quality Level
packaging
pkg of 1 kit
storage condition
protect from light
technique(s)
PCR: suitable
application(s)
detection
microbiology
storage temp.
2-8°C
General description
The kit is comprised of a combination of biological agents that reliably and completely eliminate mycoplasma contamination. The initial treatment of this eradication procedure is adequate for mycoplasma elimination in most applications. The second step suppresses and inactivates any remaining mycoplasma using a follow-up antibiotic treatment.
Application
The LookOut® Mycoplasma Elimination Kit has been developed to quickly and efficiently eliminate mycoplasma contamination from cell cultures.
Features and Benefits
- Minimal cytotoxic properties of the reagents
- Highly effective
- Retention of normal cell characteristics
Suitability
The LookOut® Mycoplasma Elimination Kit is suitable for the elimination of Mollicutes and related organisms (Mycoplasma, Acholeplasma, Spiroplasma, and Entomoplasma) in cell and virus cultures. It is not effective for treating mycoplasma contamination in Chlamydia cultures.
Legal Information
LookOut is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
The product is not intended for use as a biocide under global biocide regulations, including but not limited to US EPA′s Federal Insecticide Fungicide and Rodenticide Act, European Biocidal Products Regulation, Canada’s Pest Management Regulatory Agency, Turkey’s Biocidal Products Regulation, Korea’s Consumer Chemical Products and Biocide Safety Management Act (K-BPR) and others.
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Referencia del producto
Descripción
Precios
Storage Class
10 - Combustible liquids
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Certificados de análisis (COA)
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Los clientes también vieron
Optimized PCR-based Detection of Mycoplasma
Dobrovolny PL and Bess D
Journal of Visualized Experiments, 52 (2011)
Artículos
Know when to use antibiotics to prevent bacterial or fungal, mycoplasma, or viral contamination in cell culture and find suitable antibiotics or other biological agents.
Know when to use antibiotics to prevent bacterial or fungal, mycoplasma, or viral contamination in cell culture and find suitable antibiotics or other biological agents.
Know when to use antibiotics to prevent bacterial or fungal, mycoplasma, or viral contamination in cell culture and find suitable antibiotics or other biological agents.
Know when to use antibiotics to prevent bacterial or fungal, mycoplasma, or viral contamination in cell culture and find suitable antibiotics or other biological agents.
Protocolos
Mycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.
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How does the mechanism of action (MOA) of Product No. MP0030, LookOut® Mycoplasma Elimination Kit, differ from that of BM cyclin?
1 answer-
Helpful?
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What antibiotics are included in MP0030, the LookOut® Mycoplasma Elimination Kit? Are fluoroquinolones, macrolides, or tetracyclines part of the kit?
1 answer-
The MP0030 product contains two mycoplasma-active antibiotics, with one of them being a fluoroquinolone.
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