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MDQ1

Sigma-Aldrich

Imprint® Methylated DNA Quantification Kit

To measure global DNA methylation shifts from as low as 10 ng DNA

Sinónimos:

DNA Methylation Quantification, DNA methylation shift

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About This Item

Código UNSPSC:
41116158
NACRES:
NA.54

Descripción general

Imprint™ Methylated DNA Quantification technology provides a rapid and reliable method to measure global DNA methylation shifts. The Imprint Methylated DNA Quantification Kit contains all reagents required to detect relative levels of methylated DNA. Up to 200 ng of purified DNA is bound to the wells of the assay strip. The methylated DNA is detected using the Capture and Detection antibodies, then quantified colorimetrically. The amount of methylated DNA present in the sample is proportional to the absorbance measured.

Aplicación

Imprint® Methylated DNA Quantification Kit has been used in global DNA methylation analysis.

Características y beneficios

  • ELISA based format
  • Procedure completes in 4 easy to follow steps
  • All reagents supplied including control methylated DNA
  • Detection limit is 5 ng of fully methylated DNA
  • Input DNA may be as low as 10-200 ng
  • Procedure completes in 3.5 hours
  • 96 well format allows option of studying single samples or high-throughput studies
  • Optimized antibody and reagents with high specificity to 5-mC; no cross-reactivity to unmethylated cytosine
  • May be used with adherent and suspension cells, tissues, plasma and other body fluids such as saliva, urine and serum

Información legal

Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS
  • M8570Methylated Control DNA, 50 ng/μLSDS

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Pictogramas

CorrosionExclamation mark
GHS05,GHS07

Palabra de señalización

Warning

Frases de peligro

H290,H317,H412

Clasificaciones de peligro

Aquatic Chronic 3 - Met. Corr. 1 - Skin Sens. 1

Código de clase de almacenamiento

8A - Combustible corrosive hazardous materials

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Certificados de análisis (COA)

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Rafael Guerrero-Preston et al.
Epigenetics, 5(6), 539-546 (2010-06-05)
Environmental exposures in-utero may alter the epigenome, thus impacting chromosomal stability and gene expression. We hypothesized that in utero exposures to maternal smoking and perfluoroalkyl compounds (PFCs) are associated with global DNA hypomethylation in umbilical cord serum. Our objective was
Magdalena Gryzinska et al.
Biochemical genetics, 51(7-8), 554-563 (2013-04-05)
DNA methylation is an epigenetic modification that plays an important role in the normal development and function of organisms. The level of DNA methylation is species-, tissue-, and organelle-specific, and the methylation pattern is determined during embryogenesis. DNA methylation has
Resistance training and redox homeostasis: Correlation with age-associated genomic changes
Dimauro I, et al.
Redox Biology, 10, 34-44 (2016)
Kazuhisa Nakano et al.
Journal of immunology (Baltimore, Md. : 1950), 190(3), 1297-1303 (2013-01-02)
Rheumatoid arthritis (RA) is a chronic inflammatory disease in which fibroblast-like synoviocytes (FLS) exhibit an aggressive phenotype. Although the mechanisms responsible are not well defined, epigenetic determinants such as DNA methylation might contribute. DNA methyltransferases (DNMTs) are critical enzymes that
Felicitas Thol et al.
Journal of clinical oncology : official journal of the American Society of Clinical Oncology, 29(21), 2889-2896 (2011-06-15)
To study the incidence and prognostic impact of mutations in DNA methyltransferase 3A (DNMT3A) in patients with acute myeloid leukemia. A total of 489 patients with AML were examined for mutations in DNMT3A by direct sequencing. The prognostic impact of
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