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MAK137

Sigma-Aldrich

Phospholipase D Activity Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Sinónimos:

PLD Activity Assay Kit

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.84

uso

sufficient for 100 colorimetric or fluorometric tests

método de detección

colorimetric
fluorometric

enfermedades relevantes

cancer

temp. de almacenamiento

−20°C

Información sobre el gen

human ... PLD1(5337) , PLD2(5338) , PLD3(23646) , PLD4(122618) , PLD5(200150) , PLD6(201164)
mouse ... PLD1(18805) , PLD2(18806) , PLD3(18807) , PLD4(104759) , PLD5(319455) , PLD6(194908)
rat ... PLD1(25096) , PLD2(25097) , PLD3(361527) , PLD4(362792) , PLD5(289270) , PLD6(287366)

Categorías relacionadas

Descripción general

Phospholipase D (PLD) catalyzes the hydrolysis of the phosphodiester bond of glycerophospholipids to generate phosphatidic acid and choline. It is ubiquitously expressed and is implicated in immune function and cancer inhibition. Abnormalities in PLD expression have been associated with human cancers such as breast, renal, gastric and colorectal cancer.

Aplicación

Phospholipase D (PLD) Activity Assay Kit has been used to measure PLD activity in the liver and lysosomal fractions of the mice liver.
Suitable for the detection of phospholipase D activity in biological samples. This kit can be used to study the effects of various compounds on phospholipase D activity.

Características y beneficios

Compatible with high-throughput handling systems.

Idoneidad

Suitable for the quantitative determination of phospholipase D activity and evaluation of drug effects on phospholipase D metabolism.

Principio

In this assay, PLD hydrolyzes phosphatidylcholine to choline, which is determined using choline oxidase resulting in a colorimetric (570 nm)/fluorometric (λex = 530/λem = 585 nm) product, proportional to the PLD activity in the sample. Unit definition: one unit of PLD catalyzes the formation of 1 mmole choline per minute under the assay conditions (pH 7.4).

Pictogramas

Health hazard
GHS08

Palabra de señalización

Danger

Frases de peligro

H334,H412

Consejos de prudencia

P261 - P273 - P284 - P501

Clasificaciones de peligro

Aquatic Chronic 3 - Resp. Sens. 1

Código de clase de almacenamiento

10 - Combustible liquids

Punto de inflamabilidad (°F)

188.6 °F - closed cup

Punto de inflamabilidad (°C)

87 °C - closed cup

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Certificados de análisis (COA)

Lot/Batch Number
137CE11A18
137CE08A28
137CE06A17
137CE06A17
137CE08A28

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Phospholipase D.
Su W and Michael A F
Handbook of Cell Signaling, 1167-1176 (2009)
Walid El Kayal et al.
Horticulture research, 4, 17042-17042 (2017-11-09)
Raspberry (Rubus spp.) is an economically important crop with a restricted growing season and very limited fruit shelf-life due to its extreme tenderness. In order to prolong its shelf life, an aqueous composition containing hexanal as the key active ingredient
Jeong Kuk Park et al.
IUCrJ, 6(Pt 4), 729-739 (2019-07-19)
Ependymin was first discovered as a predominant protein in brain extracellular fluid in fish and was suggested to be involved in functions mostly related to learning and memory. Orthologous proteins to ependymin called ependymin-related proteins (EPDRs) have been found to
Design of isoform-selective phospholipase D inhibitors that modulate cancer cell invasiveness.
Scott S A, et al.
Nature Chemical Biology, 5(2), 108-108 (2009)
Karthika Sriskantharajah et al.
Plants (Basel, Switzerland), 10(11) (2021-11-28)
'Honeycrisp' (Malus domestica Borkh.), a premium applecultivar, is highly susceptible to bitter pit and decline in quality during long-term storage. In order to enhance the quality, an aqueous composition containing hexanal was applied as a preharvest spray. The effects of
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