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Merck

M8316

Sigma-Aldrich

Anti-MRP2 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Sinónimos:

Anti-ABCC2, Anti-Multidrug Resistance Associated Protein 2, Anti-cMOAT, Anti-cMRP

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~190 kDa

species reactivity

rat, human

technique(s)

indirect immunofluorescence: 1:100 using paraformaldehyde-fixed HepG2 cells
indirect immunofluorescence: 1:100 using rat liver frozen sections
western blot: 1:1,000 using whole extract of HepG2 human hepatoblastoma cells

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ABCC2(1244)
rat ... Abcc2(25303)

Categorías relacionadas

General description

Multidrug Resistance-associated Protein 2 (MRP2), also called as canalicular Multispecific Organic Anion Transporter (cMOAT), is a member of the CFTR/MRP (ABCC) subfamily of the large ATP-Binding Cassette (ABC) transporter family of transmembrane proteins. MRP2 is normally expressed in the liver, gallbladder, kidney proximal tubules, placenta, duodenum and small intestine. Localization pattern appears to vary in different species. MRP2 is predominantly localized to the apical membrane of polarized cells.
Multidrug resistance associated protein 2 (MRP2) is expressed in endothelial cells of the blood-brain barrier, various carcinoma cell lines and tumors. The gene encoding MRP2 is localized on human chromosome 10q24 and consists of 32 exons.

Specificity

Additional lower bands including an approx. 175 kDa band representing an immature unglycosylated form may be detected in various extract preparations.

Immunogen

synthetic C-terminal peptide corresponding to amino acids 1528-1545 of human MRP2 conjugated to KLH.

Application

Anti-MRP2 antibody produced in rabbit has been used in:
  • immunoblotting
  • western blotting
  • immunofluorescence

Biochem/physiol Actions

Multidrug Resistance-associated Protein 2 (MRP2) transports endogenous and exogenous anionic conjugates from hepatocytes to the bile. Thus, it contributes to bile flow and plays a role in detoxification and defense against oxidative stress. Patients with the rare autosomal recessive Dubin-Johnson Syndrome develop a mild liver disease caused by MRP2 deficiency. Up regulation of MRP2 expression may be found in hepatocellular carcinomas.
Multidrug resistance associated protein 2 (MRP2) is a transporter which is involved in the efflux of various xenobiotic compounds like doxorubicin and methotrexate.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Visite la Librería de documentos

Yan Liu et al.
PloS one, 9(1), e82681-e82681 (2014-01-10)
Methotrexate (MTX) is a key agent for the treatment of childhood acute lymphoblastic leukemia (ALL). Increased MTX plasma concentrations are associated with a higher risk of adverse drug effects. ATP-binding cassette subfamily C member 2 (ABCC2) is important for excretion
A L Dzierlenga et al.
Drug metabolism and disposition: the biological fate of chemicals, 44(11), 1799-1807 (2016-09-09)
Interindividual variability in drug response in nonalcoholic steatohepatitis (NASH) can be mediated by altered regulation of drug metabolizing enzymes and transporters. Among these is the mislocalization of multidrug resistance-associated protein (MRP2)/Mrp2 away from the canalicular membrane, which results in decreased
Nonalcoholic steatohepatitis modulates membrane protein retrieval and insertion processes
Dzierlenga AL, et al.
Drug Metabolism and Disposition, 44(11), 1799-1807 (2016)
Tsukasa Kobayashi et al.
The journal of obstetrics and gynaecology research, 48(7), 1591-1606 (2022-04-22)
Jaundice is especially common in premature infant born before 35 weeks. Because the premature infant liver is not fully developed at birth it may be incomplete the bilirubin metabolism. The purpose was to evaluate the metabolism and the excretion of bilirubin
Barbara Schaarschmidt et al.
Theranostics, 8(14), 3766-3780 (2018-08-08)
Rationale: The liver is a central organ not only for metabolism but also immune function. Life-threatening infections of both bacterial and fungal origin can affect liver function but it is yet unknown whether molecular changes differ depending on the pathogen.

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