M6908

Myeloperoxidase from human leukocytes

lyophilized powder, ≥50 units/mg protein

• Sinónimos: MPO, Peroxidase, myelo
• Número de CAS: 9003-99-0
• Comisión internacional de enzimas: 1.11.2.2 (BRENDA, IUBMB)
• Número MDL: MFCD00131641
• Código UNSPSC: 12352200
• NACRES: NA.32

Propiedades

• origen biológico: human leucocytes
• Nivel de calidad: 200
• Formulario: lyophilized powder
• actividad específica: ≥50 units/mg protein
• solubilidad: H₂O: soluble; aqueous buffers such as 50 mM sodium acetate: soluble (Freezing of the enzyme in solution may result in a substantial loss of activity.)
• Nº de acceso UniProt: P05164
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• cadena SMILES: [O+H2]O[O-]
• InChI: 1S/H2O3/c1-3-2/h1-2H
• Clave InChI: JSPLKZUTYZBBKA-UHFFFAOYSA-N
• Información sobre el gen: human ... MPO(4353)

Descripción

Descripción general

Myeloperoxidase (MPO) is encoded by the gene mapped to human chromosome 17q22-24.[1] The encoded protein is a leukocyte-derived enzyme and is an integral constituent of the innate immune response. MPO belongs to the heme peroxidase superfamily. MPO is a heme protein containing iron and is highly expressed in leukocytes, neutrophils, monocytes and some subtypes of tissue macrophages.[2]

Aplicación

Myeloperoxidase (MPO) from human leukocytes has been used in MPO assay.[3][4][5]

Myeloperoxidase from human leukocytes has been used:

• as a standard to quantify the neutrophil (polymorphonuclear cell [PMN]) infiltration in lung tissue[6]
• as a neutrophil extracelullar trap (NET) component to stimulate 10⁶ dendritic cells or 10⁶ macrophages[7]
• as a standard in myeloperoxidase (MPO) assay to measure pancreatic MPO[8]

Acciones bioquímicas o fisiológicas

Myeloperoxidase (MPO) has a crucial role to play in destruction of various microorganisms and foreign cells, such as bacteria, fungi, viruses, red cells and malignant and nonmalignant nucleated cells.[9] MPO catalyzes the production of number of reactive oxidant species (ROS) that influence tissue damage during inflammation. MPO and its downstream inflammatory pathways function as a potent therapeutic target for prophylaxis of atherosclerotic cardiovascular disease.[10] MPO catalyze the synthesis of polychlorinated dibenzo(p)dioxins and furans (PCDD/F) from precursor such as chlorophenols in presence of hydrogen peroxide (H₂O₂).[3]

Otras notas

Lysosomal heme-containing protein.

Definición de unidad

One unit of myeloperoxidase will catalyze the consumption of one micromole of hydrogen peroxide and the production of ¹/₄ micromole of tetraguaiacol per minute at pH 7.0 and 25 °C

Forma física

Lyophilized from 50 mM sodium acetate buffer, pH 6.0, 0.1 M sodium chloride

Nota de análisis

Source material tested and found negative for antibody to HIV and for HBSAG.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable