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M0404

Sigma-Aldrich

Medio basal Murashige y Skoog

powder, suitable for plant cell culture, with Gamborg′s vitamins

Sinónimos:

Medio basal MS

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About This Item

MDL number:
MFCD00240976
UNSPSC Code:
12352207
NACRES:
NA.72
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Quality Level

200

form

powder

technique(s)

cell culture | plant: suitable

application(s)

agriculture

shipped in

ambient

storage temp.

2-8°C

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Application

Murashige and Skoog Basal Medium has been used:
  • to germinate the seeds for seedling growth and germination studies[1]
  • in the suspension culture of Nicotiana plumbaginifolia for transformation[2]
  • in the root culture of hairy clone A. artemisiifolia T4[3]

Formula variant

With the macro- and micronutrients as described by Murashige and Skoog (1962) and the vitamins as described by Gamborg, et al. (1968).

Media Formulation

Quantity

Formulated to contain 4.4 grams of powder per liter of medium.

Preparation Note

Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient, micronutrient and vitamin mixtures.
Murashige and Skoog medium (M0404) contains the macro- and micronutrients of M&S basal medium as described by Gamborg, et al (1968). It can be supplemented with organics and with auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.

pictograms

Flame over circleExclamation mark
GHS03,GHS07

signalword

Warning

hcodes

H272,H319

Hazard Classifications

Eye Irrit. 2 - Ox. Sol. 3

Storage Class

5.1B - Oxidizing hazardous materials

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
SLCP3049
SLCN4595
SLCM2481
SLCP6503
SLCK9071

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Brenden Hurley et al.
PloS one, 9(12), e114921-e114921 (2014-12-17)
Pseudomonas syringae subverts plant immune signalling through injection of type III secreted effectors (T3SE) into host cells. The T3SE HopF2 can disable Arabidopsis immunity through Its ADP-ribosyltransferase activity. Proteomic analysis of HopF2 interacting proteins identified a protein complex containing ATPases
Itai Bloch et al.
Plant biotechnology journal, 19(9), 1785-1797 (2021-03-28)
The synthesis of essential amino acids in plants is pivotal for their viability and growth, and these cellular pathways are therefore targeted for the discovery of new molecules for weed control. Herein, we describe the discovery and design of small
Statistical analysis of elicitation strategies for thiarubrine A production in hairy root cultures of Ambrosia artemisiifolia
Bhagwath SG and Hjortso MA
Journal of Biotechnology, 80(2), 159-167 (2000)
Identification of a Golgi-localised GRIP domain protein from Arabidopsis thaliana
Gilson PR, et al.
Planta, 219(6), 1050-1056 (2004)
John E McLaughlin et al.
PloS one, 10(6), e0130204-e0130204 (2015-06-10)
Fusarium head blight (FHB) or scab is one of the most important plant diseases worldwide, affecting wheat, barley and other small grains. Trichothecene mycotoxins such as deoxynivalenol (DON) accumulate in the grain, presenting a food safety risk and health hazard
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Questions

1–2 of 2 Questions  

  1. What is electric conductivity of Murashige-Skoog culture solution?

    1 answer

    1. The Murashige and Skoog media products have not been tested for conductivity.

      Helpful?

  2. I would like to know if there is any problem in irradiating with UV light, media culture prepared with the medium.

    1 answer

    1. Testing protocols for plant culture media include sterilization by autoclaving. Heat-labile components are filter-sterilized and added after autoclaving, prior to asceptic dispensing to sterile vessels. UV sterilization has not been investigated as a sterilizing technique for culture media products, although this does appear to be growing in popularity. Keep in mind that UV would not melt agar used in solid media formats. Autoclaving or boiling would still be required. Please see the link below for a publication that may offer additional information:
      https://pubmed.ncbi.nlm.nih.gov/25044686/#:~:text=Ultraviolet%20(UV)%20irradiation%20is%20a,sterilization%20of%20cell%20culture%20media.

      Helpful?

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