L3397

LB Broth (Luria low salt)

Powder microbial growth medium

• Sinónimos: Luria Broth (Miller’s Modification), Luria-Bertani medium (LB)
• UNSPSC Code: 41106200
• NACRES: NA.85

Propiedades

• grade: for molecular biology
• Quality Level: 200
• sterility: non-sterile
• form: powder
• composition: NaCl, 0.5 g/L ; Tryptone, 10 g/L ; Yeast Extract, 5 g/L
• technique(s): microbiological culture: suitable
• pH: 6.8-7.2(1.55% solution)
• suitability: suitable for molecular biology; nonselective for Escherichia coli; nonselective for coliforms
• application(s): food and beverages; microbiology
• storage temp.: room temp

Descripción

General description

Luria Broth is a bacterial culture medium used for the cultivation of members of the Enterobacteriaceae especially E.coli, as well as for coliphage plaque assays. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a very low-salt formulation.

Application

LB Broth (Luria low salt) has been used as a liquid broth medium in the broth microdilution technique.

Features and Benefits

Luria′s low salt LB powder provides:

• Standard formulation
• A budget-friendly alternative to liquid
• Easy scale-up using larger package sizes

Preparation Note

1. Suspend 15.5 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121°C.

Reconstitution

Stir to suspend 15.5g powder in 1L water. Autoclave for 15 minutes at 121C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable