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Key Documents

L0543

Sigma-Aldrich

LB Agar Kanamycin-50, Plates

pre-poured LB agar plates with 50 ug/ml kanamycin

Sinónimos:

kanamycin agar plates

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About This Item

UNSPSC Code:
41121800

grade

for molecular biology

sterility

sterile; autoclaved

form

plates (10 cm)
plates (10cm, 20-25ml each)

capacity

20-25 mL

storage temp.

2-8°C

suitability

selective for Escherichia coli
selective for coliforms

General description

Miller′s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.

Application

Suitable for selective cultivation of E. coli strains that contain plasmids conferring kanamycin resistance. The bacteria can be used for DNA plasmid production or production of recombinant proteins. LB Agar Kanamycin-50, Plates has been used in the screening of Agrobacterium tumefaciens and E.coli JM83 strain with pGD103 plasmid.

Features and Benefits

Miller′s LB Agar plates provide:
  • Convenient, ready-to-use 10cm format
  • Standard formulation plus antibiotic

Components

15g/L Agar
10g/L Tryptone
10g/L NaCl
5g/L Yeast Extract
50μg/ml kanamycin

related product

Referencia del producto
Descripción
Precios

K1377

Canamicina sulfate from Streptomyces kanamyceticus, powder, BioReagent, suitable for cell culture, suitable for plant cell culture

L3147

LB Broth with agar (Miller), Highly-referenced nutrient-rich microbial growth powder medium with Agar, suitable for regular E.coli culture.

L5542

LB Agar Plates, pre-poured LB agar plates for E. coli

L5667

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L0418

LB Agar Carbenicillin - 100, Plates, pre-poured agar plates with 100 μg/ml carbenicillin

L0168

LB Agar Ampicillin - 50, Plates, pre-poured LB agar plates with 50 μg/ml ampicillin

CMC0001

SIG10 Chemically Competent Cells, for protein expression and DNA plasmid production

CMC0007

SIG10 5a Chemically Competent Cells, for DNA plasmid production

CMC0014

BL21(DE3) Chemically Competent Cells, for protein expression

CMC0017

OverExpress™ C41(DE3) Chemically Competent Cells, for the highest protein expression

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Cloning of genes encoding a 15,000-dalton peptidoglycan-associated outer membrane lipoprotein and an antigenically related 15,000-dalton protein from Haemophilus influenzae.
Deich RA, et al.
Journal of Bacteriology, 170(2), 489-498 (1988)
Agrobacterium-mediated transformation and insertional mutagenesis in Colletotrichum acutatum for investigating varied pathogenicity lifestyles
Talhinhas P, et al.
Molecular Biotechnology, 39(1), 57-67 (2008)

Artículos

Protein Expression Systems

Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.

Protein Expression Systems

Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.

Protein Expression Systems

Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.

Protein Expression Systems

Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.

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