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GLN1

Sigma-Aldrich

Glutamine and Glutamate Determination Kit

Sinónimos:

L-glutamine enzymatic quantification kit

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

usage

 kit sufficient for 50 tests

packaging

pkg of 1 kit

storage condition

dry at room temperature

technique(s)

UV/Vis spectroscopy: suitable

λmax

340 nm

application(s)

cell analysis
detection

storage temp.

2-8°C

Application

For enzymatic determination of L-glutamine and L-glutamate via reduction of NAD to NADH, measured spectrophotometrically at 339 nm.
The detection ranges for this kit are: 0.056 - 0.56 mM L-glutamate; 0.14 - 1.4 mM L-glutamine.
Glutamine and Glutamate Determination Kit has been used to determine the concentration of L-glutamine:
  • in minimum essential medium (MEM) medium
  • in the supernatant
  • in plasma

Biochem/physiol Actions

The Glutamine/Glutamate Determination Kit is designed for the spectrophotometric measurement of L-glutamine and/or L-glutamate via enzymatic deamination of L-glutamine and dehydrogenation of L-glutamate with conversion of NAD+ to NADH. This kit may be used for the spectrophotometric measurement of L-glutamine and/or L-glutamate in liquid preparations.

signalword

Danger

Hazard Classifications

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1B - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Ruiqi Liu et al.
Cancer letters, 443, 34-46 (2018-12-07)
Gankyrin plays important roles in tumorigenicity and metastasis of hepatocellular carcinoma (HCC). We have for the first time investigated the effects of Gankyrin on glycolysis and glutaminolysis both in vitro and in vivo, including in patient-derived xenografts. We reported Gankyrin
L-Glutamine and L-glutamate: UV-method with glutaminase and glutamate dehydrogenase.
Lund, P. et al.
Methods of Enzymatic Analysis, 8, 357-363 (1986)
Monitoring for overreaching in rugby league players
Coutts AJ, et al.
European Journal of Applied Physiology, 99(3), 313-324 (2007)
Wei Shi et al.
Oncology letters, 14(6), 6881-6887 (2017-11-23)
Glycolysis and glutaminolysis are heavily involved in the metabolic reprogramming of cancer cells. The activation of oncogenes and inactivation of tumor suppressor genes has a marked effect on the cellular metabolic processes glycolysis and glutaminolysis. N-Myc downstream-regulated gene 2 (NDRG2)
Jing Li et al.
Journal of cellular and molecular medicine, 23(8), 5632-5641 (2019-06-19)
Immunity imbalance and barrier damage in the intestinal mucosa are the main pathogenic factors of Crohn's disease (CD). Bis-2-(5-phenylacetamido-1,2,4-thiadiazol-2-yl) ethyl sulfide (BPTES) is a glutaminase 1 (Gls1) inhibitor with the dual functions of increasing glutamine levels and immune regulation. In
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