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GE28-9520-96

HiTrap® Con A 4B

Cytiva 28-9520-96, pack of 5 × 5 mL

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About This Item

UNSPSC Code:
23151816
NACRES:
NA.56

ligand

concanavalin A (con A)

packaging

pack of 5 × 5 mL

manufacturer/tradename

Cytiva 28-9520-96

storage condition

(100% Isopropanol)
 (20% Ethanol containing 0.1 M Acetate Buffer pH 6, 1 M NaCl, 1 mM CaCl2, 1 mM MnCl2 and 1 mM MgCl2)

parameter

42 psi

bed size

16 mm × 25 mm

bed volume

5 mL

column I.D.

16 mm

matrix

4% agarose

particle size

45-165 μm

average diameter

90 μm

cleaning

4-9

working range

4-6

storage temp.

2-8°C

Categorías relacionadas

General description

HiTrap® Con A 4B is a ready-to-use column prepacked with Con A Sepharose™ 4B, a medium for convenient separation and purification of glycoproteins, polysaccharides, and glycolipids.

Concanavalin A (Con A) is a tetrameric metalloprotein isolated from Canavalia ensiformis (jack bean). Con A binds molecules containing α-D-mannopyranosyl, α-D-glucopyranosyl, and sterically related residues. The binding sugar requires the presence of C-3, C-4 and C-5 hydroxyl groups for reaction with Con A. Con A coupled to Sepharose™ is routinely used for separation and purification of glycoproteins, polysaccharides, and glycolipids.

Features and Benefits

  • Con A Sepharose™ is concanavalin A coupled to Sepharose™ 4B.
  • HiTrap® Con A 4B columns can be operated with a syringe, a peristaltic pump, or a liquid chromatography system such as AKTA design.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

related product

Referencia del producto
Descripción
Precios

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Storage Class

3 - Flammable liquids

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

Converting from Flow Velocity to Volumetric Flow Rates

This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.

Protocolos

Purification or Removal of Glycoproteins and Polysaccharides

This page shows how to purify or remove glycoproteins and polysaccharides with Con A Sepharose 4B, Lentil Lectin Sepharose 4B, Capto Lentil Lectin from Cytiva.

Column Cleaning for Ion Exchange Chromatography and Chromatofocusing

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico