FG0100
Kits y reactivos de triglicéridos y glicerol libre
1 kit sufficient for 1000 reactions
Sinónimos:
Triglyceride and Free Glycerol Kits and Reagents
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.26
Productos recomendados
usage
kit sufficient for 1000 reactions
detection method
colorimetric
storage temp.
2-8°C
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General description
Glycerol, also referred to as glycerin or glycerine, is a 3 carbon sugar alcohol that forms the backbone of fatty acids. It is a central component for synthesis of all lipids, and acts as a backbone for triglycerides and phospholipids, which play an important role in cell membrane structure. Due to its low toxicity, glycerol is widely used in pharmaceutical, food and cosmetic formulations and is the main waste by-product of biodiesel production via transesterification. In addition to kits, the individual reagents and glycerol standard are available separately when fewer reactions are needed.
Application
Los kits de triglicéridos y de glicerol libre son para la determinación cuantitativa de glicerol, triglicéridos totales o triglicéridos libres.
The Free Glycerol Determination Kit has been used to determine the level of glycerol released into the medium from mammalian cells grown in vitro .
The Free Glycerol Determination Kit measures free, endogenous glycerol using coupled enzyme reactions and does not include initial lipase hydrolysis. Glycerol is phosphorylated by adenosine-5′-triphosphate (ATP) forming glycerol-1-phosphate (G-1-P) and adenosine-5′-diphosphate (ADP) in the reaction catalyzed by glycerol kinase (GK). G-1-P is then oxidized by glycerol phosphate oxidase (GPO) to dihydroxyacetone phosphate (DAP) and hydrogen peroxide (H2O2). Peroxidase catalyzes the coupling of H2O2 with 4-aminoantipyrine (4-AAP) and sodium N-ethyl-N-(3-sulfopropyl) m-anisidine (ESPA) to produce a quinoneimine dye that shows an absorbance maximum at 540 nm. The increase in absorbance at 540 nm is directly proportional to the free glycerol concentration of the sample.
Suitability
Suitable for the quantitative enzymatic determination of glycerol in serum or plasma.
Linkage
Además de los kits, pueden obtenerse los reactivos individuales y el patrón de glicerol por separado cuando se precisan menos reacciones.
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2
Storage Class
10 - Combustible liquids
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