Both F9765 and F4767 are suitable for determining cholesterol in cells. The F4767, Filipin III, is purified from Filipin. As it has a higher purity, it may offer a higher signal-to-noise ratio resulting in less background. These product have not been compared in side by side testing. It may be helpful to view the peer-reviewed knowledge articles to help with the appropriate selection:
F9765
Filipin complex
powder, ≥70% (UV)
Sinónimos:
Filimarisin
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About This Item
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Nombre del producto
Filipin complex from Streptomyces filipinensis, ≥70% (UV)
origen biológico
Streptomyces sp. (Streptomyces filipinensis)
Nivel de calidad
Ensayo
≥70% (UV)
Formulario
powder
solubilidad
methanol: 9.80-10.20 mg/mL, clear to hazy, yellow to very deep greenish-yellow
espectro de actividad antibiótica
fungi
Modo de acción
cell membrane | interferes
temp. de almacenamiento
−20°C
InChI
1S/C35H58O11/c1-4-5-11-16-31(42)34-33(44)22-29(40)20-27(38)18-25(36)17-26(37)19-28(39)21-32(43)23(2)14-12-9-7-6-8-10-13-15-30(41)24(3)46-35(34)45/h6-10,12-15,24-34,36-44H,4-5,11,16-22H2,1-3H3/b7-6+,10-8+,12-9+,15-13+,23-14+/t24-,25+,26-,27+,28-,29+,30+,31-,32+,33+,34-/m1/s1
Clave InChI
IMQSIXYSKPIGPD-YQRUMEKGSA-N
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Descripción general
Chemical structure: macrolide
Aplicación
Acciones bioquímicas o fisiológicas
Filipin is a polyene macrolide antibiotic and antifungal. The antifungal mechanism of action is unclear but may be due to altering membrane permeability and associated functions via binding to membrane sterols. Filipin binds to membrane sterols such as cholesterol, and it both inhibits prion protein (PrP) endocytosis and causes the release of PrP from the plasma membrane.
Filipin is a polyene macrolide antibiotic and antifungal. The antifungal mechanism of action is unclear but may be due to altering membrane permeability. Filipin binds to model crystalline phase lipid bilayers containing cholesterol, and to liquid phase bilayers with or without cholesterol, in an orientation that forms membrane pores.It both inhibits prion protein (PrP) endocytosis and causes the release of PrP from the plasma membrane.
Otras notas
Filipin contains 8 isomeric components, with filipin III as the major component.
Producto relacionado
Referencia del producto
Descripción
Precios
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Órganos de actuación
Respiratory system
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Which filipin is better to visualize cholesterol in hela cells by immunofluorescence - F9765 or F4767?
1 answer-
Helpful?
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What is the stability of filipin when dissolved in DMSO and stored at -20 degrees C (how long can it be stored like this)?
1 answer-
This product has not been tested for stability in solution, however, product SAE0088 is a prepared 5/mg/mL solution in DMSO. This product is stable for at least 2 years at -20 deg. C. Similar stability would be expected for the F9765. See the link below to the product information sheet on the SAE0088 solution:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/973/858/sae0088pis.pdfSee the link below for a sample Certificate of Analysis for the prepared solution which includes the retest date:
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/SAE0088-1ML0000207634.pdfHelpful?
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What is the difference between Filipin products F9765 and F4767?
1 answer-
The product F9765 consists of a filipin complex. It is composed of 8 isomeric components, of which filipin III is the major component in the complex. F4767 consists of filipin III alone and is considered to be a better choice for staining.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How should Product F9765, Filipin complex from Streptomyces filipinensis, be solubilized?
1 answer-
This product has low solubility in aqueous buffers. It should initially be reconstituted in DMSO at 1 mg/mL and then diluted into saline or an aqueous buffer of choice.
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What is the stability of stock solutions Product F9765, Filipin complex from Streptomyces filipinensis?
1 answer-
Solutions of filipin in aqueous or organic solvents are not considered to be stable and it is recommended that they be prepared fresh and not stored as stock solutions.
Helpful?
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