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E5134

Sigma-Aldrich

Ácido etilenodiaminotetraacético disodium salt dihydrate

Ethylenediaminetetraacetic acid disodium salt dihydrate

suitable for electrophoresis, for molecular biology, 99.0-101.0% (titration)

Sinónimos:

EDTA disodium salt, EDTA-Na2, Edatamil, Edetato disodium salt dihydrate, Etilenodiaminotetraacetato de disodio dihydrate, Sequestrene Na2

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About This Item

Fórmula empírica (notación de Hill):
C10H14N2Na2O8 · 2H2O
Número de CAS:
6381-92-6
Peso molecular:
372.24
Beilstein:
3900609
Número CE:
205-358-3
Código UNSPSC:
12352107
ID de la sustancia en PubChem:
24894605
NACRES:
NA.31
En este momento no podemos mostrarle ni los precios ni la disponibilidad

grado

for molecular biology

Nivel de calidad

300

Ensayo

99.0-101.0% (titration)

Formulario

powder

idoneidad de la reacción

reagent type: chelator

técnicas

electrophoresis: suitable

impurezas

≤0.005% Insolubles
≤0.1% Nitrilotriacetic acid (NTA)

mp

248 °C (dec.) (lit.)

solubilidad

3  M NaOH: 1.6 g/10 mL

trazas de catión

Fe: ≤0.005%
Pb: ≤0.002%

idoneidad

suitable for electrophoresis
suitable for molecular biology

actividad extraña

DNase, RNase, NICKase and protease, none detected

temp. de almacenamiento

room temp

cadena SMILES

O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O

InChI

1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;2*1H2/q;2*+1;;/p-2

Clave InChI

OVBJJZOQPCKUOR-UHFFFAOYSA-L

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Descripción general

EDTA disodium salt dehydrate chelates metal ions, forming an octahedral complex with divalent cations. This is useful in molecular biology research to inhibit enzymes dependent on activation by metal ions. In diagnostic assays, it acts as an anticoagulant during blood collection for hematological tests[1]

Aplicación

Quelante de cationes divalentes. Inhibe las enzimas, como las metaloproteasas, que requieren cationes divalentes para ser activas.
EDTA disodium salt has been used in research:
  • as a component in buffer solutions for electrophoresis, cell culture, enzymatic assays, siRNA, flow cytometry, dimetylmethylene blue, and more[2]
  • an anticoagulant for whole blood collection[1]
  • a stabilizer solution for freezing cell culture samples[3]
  • in combination with PBS and trypsin, to trypsinize cells[4]
  • for phytoextraction of heavy metals in polluted soils[5]

Características y beneficios

  • Molecular Biology grade, suitable for many molecular biology applications
  • Free of endonucleases DNase, RNase, Nickase, and Protease

Otras notas

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Precios

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E4884

Ácido etilenodiaminotetraacético disodium salt dihydrate, ACS reagent, 99.0-101.0%

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Ácido etilenodiaminotetraacético disodium salt dihydrate, meets analytical specification of Ph. Eur., BP, USP, FCC, 99.0-101.0%

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Ácido etilenodiaminotetraacético disodium salt dihydrate, BioUltra, for molecular biology, ≥99.0% (T)

Pictogramas

Exclamation markHealth hazard
GHS07,GHS08

Palabra de señalización

Warning

Frases de peligro

H332 - H373

Clasificaciones de peligro

Acute Tox. 4 Inhalation - STOT RE 2 Inhalation

Órganos de actuación

Respiratory Tract

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000416116
0000416117
0000425101
0000425092
0000425092

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Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

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Ana Amaral et al.
Animals : an open access journal from MDPI, 11(1) (2021-01-21)
Neutrophils can originate neutrophil extracellular traps (NETs). Myeloperoxidase (MPO) is a peroxidase found in NETs associated to equine endometrosis and can be inhibited by 4-aminobenzoic acid hydrazide (ABAH). Metallopeptidases (MMPs) participate in extracellular matrix stability and fibrosis development. The objectives
External quality assessment of methylmalonic acid and total homocysteine.
M?ller J, et al.
Clinical Chemistry, 45(9), 1536-1542 (1999)
Chelant-enhanced heavy metal uptake by Eucalyptus trees under controlled deficit irrigation
Fine P et al.
The Science of the Total Environment, 493, 995-1005 (2014)
External quality assessment of methylmalonic acid and total homocysteine
Clinical Chemistry, 45, 1536-1542 (1999)
Erwin G Zoetendal et al.
Nature protocols, 1(2), 954-959 (2007-04-05)
The human gastrointestinal (GI) tract contains a complex microbial community that consists of numerous uncultured microbes. Therefore, nucleic-acid-based approaches have been introduced to study microbial diversity and activity, and these depend on the proper isolation of DNA, rRNA and mRNA.
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Questions

1–7 of 7 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  3. Product E5134 has a re-test date of Aug. 2023. Has the re-test been completed?

    1 answer

    1. The specific lot number is required to determine the retest status. You may obtain this lot status by contacting our Technical Service team. We kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice, click on "Product Technical Inquires" under the Products Section with all the required information, including the specific lot number, so that a member of our team can reach out to you to assist further. Thank you.

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  4. Can solutions of Product E5134, EDTA disodium salt, be autoclaved?

    1 answer

    1. Yes.

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  5. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  6. What is the working concentration for Product E5134, EDTA disodium salt, for use as an anti-coagulant?

    1 answer

    1. The optimal concentration is 1.5 mg per mL of blood.

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  7. Is Product E5134, EDTA disodium salt, stable in solution?

    1 answer

    1. Yes. A 0.5 M stock solution at pH 8.5 is stable for months at 2-8°C.

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