D3320

Anti-DVL1 (C-terminal) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• Sinónimos: Anti-Dishevelled-1, Anti-Dsh homolog 1, Anti-Segment polarity protein dishevelled homolog
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• conjugado: unconjugated
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• Formulario: buffered aqueous solution
• mol peso: antigen ~85 kDa
• reactividad de especies: human
• validación mejorada: recombinant expression; Learn more about Antibody Enhanced Validation
• concentración: ~1.5 mg/mL
• técnicas: western blot: 2.0-4.0 μg/mL using HEK-293T cell lysate expressing human DVL1
• Nº de acceso UniProt: O14640
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... DVL1(1855)

Descripción

Descripción general

The three DVL isoforms display high sequence homology and have conserved DIX, PDZ and DEP domains required for GSK3β inactivation. DVL1 is highly expressed in adult and fetal tissues such as skeletal muscle and pancreas but is also found in the brain and neural tube.

Aplicación

Anti-DVL1 (C-terminal) antibody produced in rabbit has been used in immunoblotting and immunofluorescence.

Acciones bioquímicas o fisiológicas

Dishevelled (Dsh, DVL) operates by up-regulating β-catenin levels and stimulating T-cell factor (TCF)/lymphoid enhancer-binding factor 1 (LEF-1)-dependent transcription. In mammals, three genes encoding isoforms of dishevelled are present, DVL1, DVL2, and DVL3, that differentially mediate the wingless (wnt) canonical signaling pathway.

Dishevelled (Dsh, DVL) proteins are part of a multigene family that mediate wnt signaling pathways that are essential for the regulation of cellular proliferation, differentiation, motility, and morphogenesis. DVL1 regulates the activity of JNK (c-Jun N-terminal kinase) and GSK3β in the wnt signaling pathway. Upon activation of the wnt signaling pathway, DVL1 inactivates GSK3β through complex formation with APC (Antigen-presenting cell), β-catenin and axin proteins, releasing β-catenin from degradation. In neurons, DVL1 is localized to axonal microtubules and stabilizes microtubules by inhibiting the GSK3β mediated phosphorylation of MAP-1B.

Forma física

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)