Saltar al contenido
Merck
Todas las fotos(1)

Key Documents

View All Documentation

CYTOCOX1

Sigma-Aldrich

Cytochrome c Oxidase Assay Kit

sufficient for 100 tests, soluble and membrane bound mitochondria

Sinónimos:

Cytochrome c Oxidase Activity Assay Kit

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(1)

About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

Quality Level

200

usage

sufficient for 100 tests

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... COX1(4512)

Application

The Cytochrome c Oxidase Assay Kit uses an optimized colorimetric assay based on observation of the decrease in absorbance of ferrocytochrome c measured at 550 nm, which is caused by its oxidation to ferricytochrome c by cytochrome c oxidase. This kit is suitable for the detection of mitochondrial outer membrane integrity/mitochondrial stress and for the detection of mitochondria in subcellular fractions.

Biochem/physiol Actions

Cytochrome c oxidase [EC 1.9.3.1] is located on the inner mitochondrial membrane dividing the mitochondrial matrix from the intermembrane space, and has traditionally been used as a marker for this membrane. It is also located in the cytoplasmic membrane of bacteria. Cytochrome c oxidase provides energy for the cell by coupling electron transport through the cytochrome chain with the process of oxidative phosphorylation.

Features and Benefits

  • Simple, optimized protocol - Obtain reproducible results without special training needs
  • Useful for determining cytochrome c activity from any mitchondrial source - The enzyme is present in all mitochondria regardless of species
  • Useful for detecting the presence of mitochondria in subcellular fraction - Save time and increase confidence in the quality of organelle preparations
  • May be used in conjunction with the MITOISO1 Mitochondrial Isolation Kit - Standardized mitochondrial preparation and analysis ensures reproducibility
  • Use to analyze the intactness of mitochondrial membranes
  • Simple colorimetric measurement of solution

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS
  • C2506Cytochrome c from equine heart, ≥95% (SDS-PAGE) 50 mgSDS
  • D4641n-Dodecyl β-D-maltoside, ≥98% (GC) 10 mgSDS

pictograms

Corrosion
GHS05

signalword

Danger

hcodes

H315,H318

Hazard Classifications

Eye Dam. 1 - Skin Irrit. 2

Storage Class

10 - Combustible liquids

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Hikaru Miyagi et al.
The Journal of biological chemistry, 284(12), 7553-7560 (2009-01-23)
Cells of the yeast Saccharomyces cerevisiae contain three NAD kinases; namely, cytosolic Utr1p, cytosolic Yef1p, and mitochondrial Pos5p. Previously, the NADH kinase reaction catalyzed by Pos5p, rather than the NAD kinase reaction followed by the NADP(+)-dependent dehydrogenase reaction, had been
Filip J Larsen et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 25(8), 2843-2852 (2011-05-18)
The basal metabolic rate (BMR) is referred to as the minimal rate of metabolism required to support basic body functions. It is well known that individual BMR varies greatly, even when correcting for body weight, fat content, and thyroid hormone
Filip J Larsen et al.
Cell metabolism, 13(2), 149-159 (2011-02-03)
Nitrate, an inorganic anion abundant in vegetables, is converted in vivo to bioactive nitrogen oxides including NO. We recently demonstrated that dietary nitrate reduces oxygen cost during physical exercise, but the mechanism remains unknown. In a double-blind crossover trial we
Chao-Chin Liu et al.
Frontiers in microbiology, 11, 170-170 (2020-03-03)
Staphylococcus aureus spreads rapidly on the surface of soft agar medium. The spreading depends on the synthesis of biosurfactants, i.e., phenol soluble modulins (PSMs), which facilitate colony spreading of S. aureus. Our earlier study demonstrated that water accumulates in a
Uta Keil et al.
The Journal of biological chemistry, 279(48), 50310-50320 (2004-09-17)
Increasing evidence suggests an important role of mitochondrial dysfunction in the pathogenesis of Alzheimer's disease. Thus, we investigated the effects of acute and chronic exposure to increasing concentrations of amyloid beta (Abeta) on mitochondrial function and nitric oxide (NO) production
Ver todos los artículos relacionados

Artículos

Organelle Isolation

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

Organelle Isolation

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

Organelle Isolation

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

Organelle Isolation

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

Ver todo

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico