CE0050
CelLytic™ MEM Protein Extraction Kit
Sinónimos:
Membrane protein extraction kit
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About This Item
Código UNSPSC:
41116134
NACRES:
NA.56
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Productos recomendados
técnicas
protein extraction: suitable
Nivel de calidad
Condiciones de envío
wet ice
temp. de almacenamiento
−20°C
Descripción general
CelLytic™ MEM Protein Extraction Kit offers a fast and convenient method to isolate hydrophobic and raft microdomain associated proteins from cells. The method is based on phase separation and does not require cell membrane isolation. The separated proteins can be used for further experiments such as SDS-PAGE, Western blotting, dot blotting, and immunoprecipitation. The kit has been tested on, but not limited to, HeLa, HEK-293, NIH 3T3, COS and CHO cell lines.
Membrane proteins make up around 20-30% of an organism′s genome and serve as cellular gatekeepers, regulators, and sensors. They have diverse cellular functions, such as shielding the cell from external toxins, being the starting point of intracellular signaling cascades, and retaining critical ion concentrations.[1]
Aplicación
Idoneidad
Sufficient reagents supplied for 80 tests.
Información legal
CelLytic is a trademark of Sigma-Aldrich Co. LLC
Solo componentes del kit
Referencia del producto
Descripción
- Lysis and Separation Buffer 50 mL
- Wash Buffer for CelLytic MEM 50 mL
- Sodium Chloride, 4M Solution 1.5 mL
Los componentes del kit también están disponibles por separado
Referencia del producto
Descripción
SDS
- P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 1 mLSDS
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Eye Irrit. 2 - Skin Irrit. 2
Código de clase de almacenamiento
10 - Combustible liquids
Punto de inflamabilidad (°F)
188.6 °F - closed cup
Punto de inflamabilidad (°C)
87 °C - closed cup
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