PORTHOS

93122323, Porcine thyroid, Epithelial

• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: PORTHOS, 93122323
• origen biológico: Porcine thyroid
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Epithelial
• productos: Thyroglobulin, plasminogen activator (u-PA)
• receptores: Thyrotropin, insulin, Insulin Growth Factor (IGF-1), hydrocortisone
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Porcine thyroid epithelial

Descripción línea celular

Derived from porcine thyroid. The cells have been adapted to grow in low serum (0.1%) media.

Aplicación

Regulation of thyroid cell metabolism

Medio de cultivo

Click:RPMI (1:1) + 10μg/ml Insulin + 10mM Hydrocortisone + 5μg/ml Transferrin + 10ng/ml glycyl-L-histydyl-L-lysine acetate + 10ng/ml somatostatin + 10mU/ml Thyroid Stimulating Hormone (TSH) + 2mM Glutamine + 0.1% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:2 seeding at 2 x 10 ⁴ cells/cm² using 0.05% trypsin/0.025% EDTA in Puck F saline; 2.5% CO₂; 37°C.

Otras notas

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