HTC

93120108, rat liver, Epithelial

• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: HTC, 93120108
• origen biológico: rat liver
• modo de crecimiento: Adherent
• cariotipo: 2n = 66, hypertriploid
• morfología: Epithelial
• productos: Not specified
• receptores: Dexamethasone phosphate; hydrocortisone
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Rat hepatoma

Descripción línea celular

Derived from a rat ascites tumour, which in turn was derived from a solid hepatoma induced in male Buffalo rats by feeding them 0.04% N,N′-2,7fluorenyle-bis-2,2,2-trifluoroacetamide for 12.4 months.

Aplicación

Tumourigenicity and enzymatic studies

Medio de cultivo

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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