TA3 Hauschka

85061102, mouse breast, Epithelial

• UNSPSC Code: 41106514

Propiedades

• product name: TA3 Hauschka, 85061102
• biological source: mouse breast
• growth mode: Adherent
• karyotype: 2n = 41
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Mouse mammary carcinoma

Cell Line Description

In form of tumorigenic ascites shows diminished expression of histocompatibility antigens and readily overcomes histoincompatibility barriers. Immunoresistant TA3/Ha sub-line arose spontaneously without subjection to any known immunological selection pressure.

Application

Tumourigenicity studies

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Cells grow partly in suspension, therefore spin down supernatant at time of subculture to collect the cells.

Other Notes

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