OX-22

Reactivity: Anti-CD45, rat, 84112013

• UNSPSC Code: 41106514

Propiedades

• biological source: Mouse x Mouse Hybridoma
• ImmunoDon: BALB/c mouse spleen
• Myeloma: NS1
• Reactivity: Anti-CD45, rat
• technique(s): cell culture | mammalian: suitable
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Mouse NS1 x Mouse BALB/c spleen

Cell Line Description

The antibody reacts mainly with high molecular weight (190-220kDa) form of leukocyte common antigen. Labels all B lymphocytes and 50% of bone marrow cells and 2% of thymocytes. The antibody is directed against CD8+ve T, NK and 75% of CD4+ve cells. Same distribution as OX-32: antibodies do not compete.

Immunogen

PHA activated rat lymph node cells

Culture Medium

RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Seed at 5x10,000 viable cells/ml. Subculture when cells reach 5x100,000-1x1,000,000 cells/ml (usually after 3-4 days); 5% CO₂; 37°C.

Other Notes

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Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.