RCC4 plus vector alone

3112702, human kidney, Epithelial

• Sinónimos: Human RCC4, RCC4 Cell Line
• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: RCC4 plus vector alone, 03112702
• origen biológico: human kidney
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Epithelial
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Renal cell carcinoma cell line RCC4 stably transfected with an empty expression vector, pcDNA3, conferring neomycin resistance.

Descripción línea celular

Renal cell carcinoma cell line RCC4 stably transfected with an empty expression vector, pcDNA3, conferring neomycin resistance. This cell line is VHL-deficient i.e. the von Hippel-Lindau tumour suppressor gene product is not expressed. These cells are tumourigenic.

Aplicación

RCC4 plus vector alone is mainly used to study the tumour suppressor gene product function - von Hippel-Lindau tumour suppressor gene. It is also used in cell responses to hypoxia.

• RCC4 plus vector alone has been used in study involving the effect of in vitro ischemia on canonical hypoxic HIF-1α (Hypoxia Inducible Factor α) stabilization.
• It has been used in the study involving CMA (chaperone-mediated autophagy)-mediated degradation of HIF1α in the lysosome.
• It has also been used to study lipodepsipeptide MDN-0066-mediated inhibition of VHL/HIF (Von Hippel-Lindau/hypoxia inducible factor) pathway.

Perfil del ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 10,12
D13S317: 12
D16S539: 11
D5S818: 12
D7S820: 8.2
THO1: 9.3
TPOX: 11
vWA: 16

Medio de cultivo

DMEM + 10% FBS + 2 mM Glutamine + G418 0.5mg/ml

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 1-3 x 10,000 cells/cm² using 0.25% Trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.