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Merck

C7611

Sigma-Aldrich

Monoclonal Anti-Caspase 12 antibody produced in rat

clone 14F7, purified immunoglobulin, buffered aqueous solution

Sinónimos:

Anti-CASP-12, Anti-CASP12P1

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.41

origen biológico

rat

Nivel de calidad

conjugado

unconjugated

forma del anticuerpo

purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

14F7, monoclonal

Formulario

buffered aqueous solution

mol peso

antigen ~50 kDa

reactividad de especies

rat, mouse

técnicas

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1-2 μg/mL using whole cell extract of rat kidney cells NRK cells

isotipo

IgG2a

Nº de acceso UniProt

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

Descripción general

Caspase 12 is a protein that belongs to a family of enzymes called caspases that cleave their substrates at C-terminal aspartic acid residues. The human caspase 12 gene is localized at a single locus within the caspase 1/ICE gene cluster on chromosome 11q22. The functionality is lost in humans and they cannot play a role in Alzheimer′s disease. Caspase-12 is detected in endoplasmic reticulum (ER), plays a role in ER-mediated apoptosis and inflammatory caspase activation pathway.
Monoclonal Anti-Caspase 12 (rat IgG2a isotype) is derived from the 14F7 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from CD rats immunized with a recombinant mouse caspase 12. Caspases (cysteine-requiring aspartate protease) are a family of proteases that share similarities in amino acid sequences, structure and substrate specificity. Caspase 12 is ubiquitously expressed in mouse tissues, at high levels in muscle, liver and kidney and at moderate levels in brain, where it is present in cortical neurons, Purkinje cells, brainstem neurons, and olfactory neurons.

Especificidad

Recognizes full length and possibly truncated p20 caspase 12.

Inmunógeno

recombinant mouse caspase 12 (residues 95-318).

Aplicación

Monoclonal Anti-Caspase 12 has been used in immunoblotting and immunocytochemistry (4% paraformaldehyde-fixed, paraffin-embedded).

Acciones bioquímicas o fisiológicas

Caspase 12 (CASP12) has a downregulatory function during infection and may play a role in protection against inflammatory disease. It is associated with rheumatoid arthritis in African-Americans. Caspase-12 inhibits caspase-1 activation and is associated with susceptibility factor for bacterial sepsis but does not influence the susceptibility to Candida sepsis.
Murine caspase 12 (CASP12) is activated during endoplasmic reticulum stress and activates other caspases which act on substrates, ultimately leading to apoptosis. The protein has been shown to be activated during progression of retinal degeneration in animal models. In mice models, CASP12 has been shown to cause neuronal death resulting from ischemia/reperfusion. Casp12 might stimulate nuclear factor-κB (NF-κB) by directing the post-translational degradation of inhibitor of kappa B (IκBα). Overexpression of caspase 12 induces apoptosis, which is inhibited by the pan caspase inhibitor carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]- fluoromethyl ketone (z-VAD-FMK).

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Nota de preparación

Purified from culture supernatant of hybridoma cells grown in a bioreactor.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Activation of the unfolded protein response in infarcted mouse heart and hypoxic cultured cardiac myocytes
Thuerauf DJ, et al.
Circulation Research, 99(3), 275-282 (2006)
Caspase 12 degrades I kappaB alpha protein and enhances MMP-9 expression in human nasopharyngeal carcinoma cell invasion
Chu WK, et al.
Testing, 8(20), 33515-33515 (2017)
Activation of caspase-12 by endoplasmic reticulum stress induced by transient middle cerebral artery occlusion in mice
Shibata M, et al.
Neuroscience, Neuroscience.(2003;118), 2-2 (2003)
Chun-Yan Wang et al.
Molecular medicine reports, 16(3), 3587-3593 (2017-07-18)
It was previously confirmed that hydrogen sulfide (H2S) has a neuroprotective effect, preventing homocysteine‑induced neurotoxicity. However, the exact molecular mechanisms underlying this protective effect remain to be fully elucidated. Endoplasmic reticulum (ER) stress contributes to homocysteine‑induced neurotoxicity. Silent mating type
Laura Marshall et al.
Immunogenetics, 66(4), 281-285 (2014-02-12)
CASPASE-12 (CASP12) has a downregulatory function during infection and thus may protect against inflammatory disease. We investigated the distribution of CASP12 alleles (#rs497116) in African-Americans (AA) with rheumatoid arthritis (RA). CASP12 alleles were genotyped in 953 RA patients and 342

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