C3956

Anti-c-Myc antibody produced in rabbit

~0.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• Sinónimos: Myc Tag Polyclonal Antibody, c-Myc Antibody
• Número MDL: MFCD00239651
• Código UNSPSC: 12352203
• NACRES: NA.56

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• Formulario: buffered aqueous solution
• reactividad de especies: human
• concentración: ~0.5 mg/mL
• técnicas: immunocytochemistry: 5-10 μg/mL; immunoprecipitation (IP): 1-2 μg using immunoprecipitates a c-Myc fusion protein from transfected mammalian cell lysates or bacterial extracts; indirect immunofluorescence: 5-10 μg/mL using detects c-Myc fusion proteins in methanol-acetone fixed transiently transfected cells; microarray: suitable; western blot: 0.5-1 μg/mL using detects c-Myc fusion proteins in cell extracts from transfected cultures as well as bacterial lysates.
• Nº de acceso UniProt: P01106
• temp. de almacenamiento: −20°C
• Información sobre el gen: human ... MYC(4609)

Descripción

Descripción general

Anti-c-Myc antibody is used for the detection of the c-Myc tag sequence on c-Myc tagged fusion proteins.This polyclonal antibody is produced in rabbit and recognizes the c-Myc sequence at the N-terminus and C-terminus. C3956 is an Affinity purified antibody, increasing sensitivity in most applications.

MYC (MYC proto-oncogene) is a transcriptional factor and oncoprotein. It is located on chromosome 8q24. c-Myc gene codes for basic helix-loop-helix/leucine zipper (bHLH/LZ) transcription factor.

Especificidad

N-terminal or C-terminal c-Myc (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) tagged fusion proteins.

Inmunógeno

peptide corresponding amino acids 408-425 of the human c-myc proto-oncogene, conjugated to maleimide-activated KLH through a C-terminal added cysteine residue.

Aplicación

Anti-c-Myc antibody has been used in western blotting and immunofluorescence.

Antibody is recommended for use in immunoblotting, immunoprecipitation and immunofluorescence.
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Acciones bioquímicas o fisiológicas

MYC (MYC proto-oncogene) may be essential for cancer initiation, promotion and therapy resistance. Overexpression of MYC in DLBCL (diffuse large B-cell lymphoma) results in poor outcome and invasive treatment when medicated with rituximab plus cyclophosphamide, doxorubicin, vincristine and prednisone (R-CHOP). The cellular myelocytomatosis (c-myc) oncogene plays a major role in cellular proliferation, differentiation and apoptosis. It acts as transcriptional regulator of gene expression.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)