C3865

Anti-N-Cadherin antibody, Mouse monoclonal

clone GC-4, purified from hybridoma cell culture

• Sinónimos: Anti-A-CAM, Anti-A-Cell Adhesion Molecule
• Número MDL: MFCD00164511
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• conjugado: unconjugated
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: GC-4, monoclonal
• Formulario: buffered aqueous solution
• mol peso: antigen 135 kDa
• reactividad de especies: chicken, monkey, mouse, human, rabbit, rat
• técnicas: electron microscopy: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: 10-20 μg/mL using rat cardiac muscle frozen sections; western blot: 10-20 μg/mL using chicken cardiac muscle or COS-7 cell extracts
• isotipo: IgG1
• Nº de acceso UniProt: P19022
• aplicaciones: research pathology
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... CDH2(1000) ; mouse ... Cdh2(12558) ; rat ... Cdh2(83501)

Descripción

Descripción general

N-Cadherin is a member of the cadherin family of proteins that mediate cell-cell adhesion in a calcium dependent manner. N-cadherin is a transmembrane glycoprotein that localizes in the cell-cell adherens junction on the plasma membrane. The protein is known to be localized in the cell membrane.

Aplicación

Anti-N-Cadherin antibody is suitable for the following procedures:

• electron microscopy
• flow cytometry
• immunocytochemistry
• immunohistochemistry at a concentration of 10-20μg/mL using rat cardiac muscle frozen sections
• western blot at a concentration of 10-20μg/mL using chicken cardiac muscle or COS-7 cell extracts

Acciones bioquímicas o fisiológicas

CDH11 gene encodes a type II classical cadherin that mediate calcium-dependent cell-cell adhesion.

Higher expression of CDH2 gene was observed in tumor cells. It is known to induce the invasive and chemoresistant attributes to the cancer cells. N-glycosylation of N-cadherin is associated with the process of tumorigenesis especially in mammary gland, renal system, and ovary. Loss of expression of the gene was observed in adrenocortical carcinoma.

Forma física

0.2 μm-filtered solution in 0.01 M phosphate buffered saline, pH 7.4.

Otras notas

Clone GC-4 is also known as GB-9.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves