Saltar al contenido
Merck
Todas las fotos(1)

Documentos

C-22020

Sigma-Aldrich

Endothelial Cell Growth Medium MV

Ready-to-use kit including Basal Medium and SupplementMix, 500 ml

Sinónimos:

Primary Cell Culture Media

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización


About This Item

UNSPSC Code:
12352207
NACRES:
NA.71

form

liquid

packaging

pkg of 500 ml

technique(s)

cell culture | mammalian: suitable

shipped in

wet ice

General description

Endothelial Cell Growth Medium MV (Ready-to-use)

Application

Endothelial Cell Growth Medium MV is a low-serum (5% V/V) medium developed for the in vitro cultivation of endothelial cells from microvascular vessels, the coronary artery, and the aorta. The media are optimized for primary human cells but can also be used for bovine, porcine, murine, rat and dog endothelial cells from microvascular vessels as well as for various endothelial cell lines.Recommended for:Human Dermal Microvascular Endothelial Cells (HDMEC)Human Coronary Artery Endothelial Cells (HCAEC)Human Aortic Endothelial Cells (HAoEC)Human Pulmonary Microvascular Endothelial Cells (HPMEC)Human Dermal Lymphatic Endothelial Cells (HDLEC)Human Dermal Blood Endothelial Cells (HDBEC)Human Cardiac Microvascular Endothelial Cells (HCMEC)Human Uterine Microvascular Endothelial Cells (HUtMEC)Note: For best transfection results we recommend using PromoCell heparin-free Endothelial Cell Growth Medium MV2. Heparin, included all our other our Endothelial Cell Growth Media formulations, may reduce transfection efficiency.

Components

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

Quality

All lots of PromoCell Endothelial Cell Media MV/MV2 are subjected to comprehensive quality control tests using primary human endothelial cells. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested endothelial cells. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Preparation Note

Final supplement concentrations after addition to the basal medium:Fetal Calf Serum: 0.05 ml / mlEndothelial Cell Growth Supplement: 0.004 ml / mlEpidermal Growth Factor (recombinant human): 10 ng / mlHeparin: 90 µg / mlHydrocortisone: 1 µg / ml

Storage and Stability

Store the Basal Medium at 4 to 8°C in the dark and the SupplementMix or SupplementPack at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete Medium and keep the remaining medium refrigerated at 4 to 8°C. Note: The SupplementMix and SupplementPack are delivered thawed and can be frozen after arrival without losing any activity.

Subculture Routine

Click here for more information.

Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Protocolos

Cell culture protocol: the endothelial cell transwell migration and invasion assay used to study angiogenesis and cancer cell metastasis. Explore over 350 PromoCell products.

Cell culture protocol: the endothelial cell transwell migration and invasion assay used to study angiogenesis and cancer cell metastasis. Explore over 350 PromoCell products.

Cell culture protocol: the endothelial cell transwell migration and invasion assay used to study angiogenesis and cancer cell metastasis. Explore over 350 PromoCell products.

Cell culture protocol: the endothelial cell transwell migration and invasion assay used to study angiogenesis and cancer cell metastasis. Explore over 350 PromoCell products.

Contenido relacionado

Cell culture protocol: the endothelial tube formation assay to study angiogenesis using HUVECs and other endothelial cell types. Explore over 350 PromoCell products.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico