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Merck

B1267

Sigma-Aldrich

Biotin-maleimide

≥95% (TLC), powder

Sinónimos:

N-Biotinoyl-N′-(6-maleimidohexanoyl)hydrazide

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About This Item

Fórmula empírica (notación de Hill):
C20H29N5O5S
Número de CAS:
Peso molecular:
451.54
Número MDL:
Código UNSPSC:
12352203
ID de la sustancia en PubChem:
NACRES:
NA.46
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Nivel de calidad

Ensayo

≥95% (TLC)

Formulario

powder

solubilidad

acetic acid: 20 mg/mL

temp. de almacenamiento

2-8°C

cadena SMILES

O=C(CCCCCN1C(=O)C=CC1=O)NNC(=O)CCCC[C@@H]2SC[C@@H]3NC(=O)N[C@H]23

InChI

1S/C20H29N5O5S/c26-15(7-2-1-5-11-25-17(28)9-10-18(25)29)23-24-16(27)8-4-3-6-14-19-13(12-31-14)21-20(30)22-19/h9-10,13-14,19H,1-8,11-12H2,(H,23,26)(H,24,27)(H2,21,22,30)/t13-,14-,19-/m0/s1

Clave InChI

VAPZWEFPQRDVHR-NJSLBKSFSA-N

Descripción general

Biotin-maleimide is a biotin specific thiol containing reagent used to biotinylate different proteins through artificially induced or natural sulphadryl group. It can be used as a universal, multipurpose, thiol-specific probe. Biotin-maleimide can also be used in dot blots to detect protein SH groups[1].

Aplicación

Biotin-maleimide- 0.01 mg/ml in PBS containing 0.1% Tween 20, incubated for 1 h at room temperature can be used to detect deprotected cysteine thiols present on immobilized peptides[2].
Sulfhydryl-specific biotinylation reagent. Couples by highly efficient thioether linkage. Typically used to couple to intrinsic sulfhydryls, e.g. protein, and avoid modification of primary amine.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)


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K Gregorius et al.
Analytical biochemistry, 299(1), 84-91 (2001-12-01)
The orientation of an immobilized antigen is important for recognition by, e.g., an antibody. When noncovalent passive adsorption is used for immobilization, the number of ways that the antigen can attach to the surface is numerous and control of how
E A Bayer et al.
Analytical biochemistry, 149(2), 529-536 (1985-09-01)
A biotin-containing, thiol-specific reagent, 3-(N-maleimido-propionyl) biocytin (MPB), was synthesized and used to biotinylate various proteins via native or artificially induced sulfhydryl groups. In combination with appropriate avidin- or streptavidin-conjugated markers (i.e., fluorescent, enzyme-conjugated, electron-dense, etc.), MPB essentially constitutes a universal
Camilla To Benfield et al.
Life science alliance, 3(1) (2019-12-13)
Host interferon-induced transmembrane proteins (IFITMs) are broad-spectrum antiviral restriction factors. Of these, IFITM3 potently inhibits viruses that enter cells through acidic endosomes, many of which are zoonotic and emerging viruses with bats (order Chiroptera) as their natural hosts. We previously
Nicolai T Sand et al.
International journal of molecular sciences, 21(7) (2020-04-05)
Early detection and monitoring of cancer progression is key to successful treatment. Therefore, much research is invested in developing technologies, enabling effective and valuable use of non-invasive liquid biopsies. This includes the detection and analysis of circulating tumor cells (CTCs)
Lichao Chen et al.
Developmental cell, 53(4), 444-457 (2020-04-25)
The redox-based protein S-nitrosylation is a conserved mechanism modulating nitric oxide (NO) signaling and has been considered mainly as a non-enzymatic reaction. S-nitrosylation is regulated by the intracellular NO level that is tightly controlled by S-nitrosoglutathione reductase (GSNOR). However, the

Questions

  1. What is the solubility of B1267 Biotin-maleimide in DMSO?

    1 answer
    1. The B1267 Biotin-maleimide is a white powder that is soluble in DMSO at 10mg/ml, resulting in a clear colorless solution. A stock solution of the product in DMSO at 20 mg/ml can be prepared without the need for heat. This solution can then be diluted 1:10 with an aqueous buffer system, such as PBS or other. Concentrations of 40-50 mg/ml can also be prepared, but heat, i.e. 40°C, is needed. For most applications, a stock of 20 mg/ml would be suitable.

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