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A6792

Anti-Dog IgG (whole molecule)−Peroxidase antibody produced in rabbit

Name: Anti-Dog IgG (whole molecule)−Peroxidase antibody produced in rabbit
Brand: SIGMA

affinity isolated antibody, buffered aqueous solution

Sinónimos:

Rabbit Anti-Dog IgG (whole molecule)−HRP

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UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00162268

Conjugate:

peroxidase conjugate

Clone:

polyclonal

Application:

ELISA (d)

Citations:

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Propiedades

biological source

rabbit

Quality Segment

200

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:10,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Descripción

General description

IgG (immunoglobulin G) antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids. Mammalian IgG has four subclasses- IgG1, IgG2a, IgG2b and IgG2c.

Specificity of the anti-dog IgG antiserum is determined by immunoelectrophoresis, prior to conjugation, versus normal dog serum and dog IgG.

Application

Anti-Dog IgG (whole molecule) Peroxidase antibody produced in rabbit has been used:

for the detection of antibodies against noro viruses, by ELISA (enzyme linked immunosorbent asay) at 1:2000 for 1 hour at 37°C
in IgG (Immunoglobulin G) staining method
in ELISA to detect the incorporation of RVGTM (a rabies virus G protein variant)

Biochem/physiol Actions

IgG (Immunoglobulin G) antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. Anti-Dog IgG antibody is a secondary antibody. Second antibodies (secondary antibodies) are raised against primary antibodies, which bind to specific antigens to create antigen antibody complexes.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin and 0.05% MIT

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in "Immunofluorescence and Related Staining Techniques," Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.