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A0855

Sigma-Aldrich

Anti-Auxin antibody, Mouse monoclonal

clone 1E11-C11, purified from hybridoma cell culture

Sinónimos:

Anti-IAA, Anti-Indole-3-acetic acid

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About This Item

MDL number:
MFCD07785405
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

recombinant

expressed in mouse cell line

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1E11-C11, monoclonal

form

PBS solution

species reactivity

plant

technique(s)

dot blot: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
indirect ELISA: 1-2 μg/mL using using IAA conjugated to BSA
western blot: suitable

isotype

IgG2b

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Auxin is a plant hormone that regulates physiological growth and development. This hormone regulates cellular functions such as elongation, turgor, division, and differentiation. Indoleacetic acid (IAA) is recognized as the main auxin in several plants. IAA is synthesized from tryptophan (Trp) using Trp dependent pathways,. Mouse monoclonal anti-auxin antibody recognizes indoleacetic acid but not free unmethylated IAA.

Immunogen

indoleacetic acid (IAA) carboxyl linked to ovalbumin.

Application

Indole-3-acetic acid was identified in developing P.abies spruce plant parrafin embedded formalin-fixed embryos by immunohistochemistry with the monoclonal anti-indole-3-acetic acid antibody.
Mouse monoclonal anti-auxin antibody has been used for immunofluorescence studies. The antibody can also be used for ELISA, dot blot and western blot analyses.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Tadashi Sakata et al.
Proceedings of the National Academy of Sciences of the United States of America, 107(19), 8569-8574 (2010-04-28)
With global warming, plant high temperature injury is becoming an increasingly serious problem. In wheat, barley, and various other commercially important crops, the early phase of anther development is especially susceptible to high temperatures. Activation of auxin biosynthesis with increased
Yolanda Pérez-Pérez et al.
International journal of molecular sciences, 24(13) (2023-07-14)
The microspore can follow two different developmental pathways. In vivo microspores follow the gametophytic program to produce pollen grains. In vitro, isolated microspores can be reprogrammed by stress treatments and follow the embryogenic program, producing doubled-haploid embryos. In the present
Aurora Alaguero-Cordovilla et al.
Plant, cell & environment, 44(5), 1642-1662 (2021-01-20)
Adventitious roots (ARs) are produced from non-root tissues in response to different environmental signals, such as abiotic stresses, or after wounding, in a complex developmental process that requires hormonal crosstalk. Here, we characterized AR formation in young seedlings of Solanum
Yin Xin et al.
Horticulture research, 11(4), uhae054-uhae054 (2024-05-06)
Lily bulbils, which serve as advantageous axillary organs for vegetative propagation, have not been extensively studied in terms of the mechanism of bulbil initiation. The functions of auxin and sucrose metabolism have been implicated in axillary organ development, but their
Gen Che et al.
Development (Cambridge, England), 147(7) (2020-03-14)
The WUSCHEL-CLAVATA3 pathway genes play an essential role in shoot apical meristem maintenance and floral organ development, and under intense selection during crop domestication. The carpel number is an important fruit trait that affects fruit shape, size and internal quality
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