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28616

Sigma-Aldrich

Atto 425-Biotin

BioReagent, suitable for fluorescence, ≥95.0% (HPCE)

Sinónimos:

Biotin-Atto 425

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About This Item

Fórmula empírica (notación de Hill):
C37H53N5O7S
Peso molecular:
711.91
MDL number:
MFCD04974818
UNSPSC Code:
12352108
eCl@ss:
34058011
PubChem Substance ID:
57648315
NACRES:
NA.32

product line

BioReagent

assay

≥95.0% (HPCE)

form

powder

manufacturer/tradename

ATTO-TEC GmbH

fluorescence

λex 437 nm; λem 483 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

CCOC(=O)C1=Cc2cc3C(C)CC(C)(C)N(CCCC(=O)NCCCCCNC(=O)CCCCC4SCC5NC(=O)NC45)c3cc2OC1=O

InChI

1S/C37H53N5O7S/c1-5-48-34(45)26-19-24-18-25-23(2)21-37(3,4)42(28(25)20-29(24)49-35(26)46)17-11-14-32(44)39-16-10-6-9-15-38-31(43)13-8-7-12-30-33-27(22-50-30)40-36(47)41-33/h18-20,23,27,30,33H,5-17,21-22H2,1-4H3,(H,38,43)(H,39,44)(H2,40,41,47)

InChI key

LPVYZZMUDCZDQO-UHFFFAOYSA-N

General description

Atto 425 is a new label with high quantum yield (0.90) as well as sufficient stoke′s shift (excitation maximum 436 nm, emission maximum 484 nm). It is optimized for excitation with argon laser, and is characterized by high photostability. It does nearly not show cis-trans-isomerization, which limits brightness and reproducibility for many other dyes.

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation. Atto 425 biotin is suitable for fluorescence labeling of avidin/streptavidin conjugated molecules.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Immunohistochemical methods for the demonstration of tumor markers.
H Denk
Current topics in pathology. Ergebnisse der Pathologie, 77, 47-69 (1987-01-01)
J Oertel et al.
Journal of cancer research and clinical oncology, 126(8), 425-440 (2000-08-29)
Immunocytochemical investigations of fixed cells are used to enhance diagnostic accuracy in haematology and oncology. The alkaline-phosphatase/anti(alkaline phosphatase) technique, immunoperoxidase and the avidin-biotin technique are the most important methods in immunocytochemistry. Tyramide-enhanced immunostaining is a new powerful technique. Peripheral blood
Katrin G Heinze et al.
Biophysical journal, 86(1 Pt 1), 506-516 (2003-12-26)
Confocal fluorescence spectroscopy is a versatile method for studying dynamics and interactions of biomolecules in their native environment with minimal interference with the observed system. Analyzing coincident fluctuations induced by single molecule movement in spectrally distinct detection channels, dual-color fluorescence
Anna I Sulatskaya et al.
The journal of physical chemistry. B, 116(8), 2538-2544 (2012-01-25)
Benzothiazole dye thioflavin T (ThT) is a sensitive probe for amyloid fibril detection. The ThT probing is based on its unique ability to form highly fluorescent complexes with amyloid and amyloid-like fibrils. In this work we propose an approach of
Triple FRET: a tool for studying long-range molecular interactions.
Elke Haustein et al.
Chemphyschem : a European journal of chemical physics and physical chemistry, 4(7), 745-748 (2003-08-07)
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