The growth rate of these cells is subject to multiple factors, including the age and origin of the cells, growth conditions, and media. While the doubling time of these cells typically falls within a range of 22 to 46 hours, it is also not uncommon to observe a variation of up to ±10 hours beyond this range.
102-05A
Human Epidermal Keratinocytes: HEK, adult
Sinónimos:
HEK cells
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About This Item
Código UNSPSC:
41106514
NACRES:
NA.81
En este momento no podemos mostrarle ni los precios ni la disponibilidad
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origen biológico
human neonatal foreskin or adult skin (normal)
Nivel de calidad
envase
pkg of 500,000 cells
fabricante / nombre comercial
Cell Applications, Inc
modo de crecimiento
Adherent
cariotipo
2n = 46
morfología
Epidermal
técnicas
cell culture | mammalian: suitable
enfermedades relevantes
allergies; cancer
Condiciones de envío
dry ice
temp. de almacenamiento
−196°C
Descripción general
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Epidermal keratinocytes comprise 90 % of all cells found in the epidermis, the outermost layer of skin. They produce keratin, the intermediate filament, largely responsible for skin’s barrier function. Through their interactions with epidermal melanocytes, keratinocytes obtain melanin and store it as a means of protection from UV radiation.
HEK provide an excellent model system to study many aspects of epithelial function and disease, particularly those related to skin biology and toxicology, as well as HPV infection.
When grown on inserts and provided with the liquid/air interface, HEK can differentiate into a stratified squamous epithelium and serve as a more physiological 3D tissue model for in vitro studies.
Epidermal keratinocytes comprise 90 % of all cells found in the epidermis, the outermost layer of skin. They produce keratin, the intermediate filament, largely responsible for skin’s barrier function. Through their interactions with epidermal melanocytes, keratinocytes obtain melanin and store it as a means of protection from UV radiation.
HEK provide an excellent model system to study many aspects of epithelial function and disease, particularly those related to skin biology and toxicology, as well as HPV infection.
When grown on inserts and provided with the liquid/air interface, HEK can differentiate into a stratified squamous epithelium and serve as a more physiological 3D tissue model for in vitro studies.
Origen línea celular
Skin
Aplicación
Skin biology and toxicology, wound healing, artificial skin, cell proliferation, migration, UV light response
Componentes
Basal Medium containing 10% FBS & 10% DMSO
Nota de preparación
- Primary culture, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 16 doublings
Rutina de subcultivo
Please refer to the HEK Culture Protocol.
Cláusula de descargo de responsabilidad
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Certificados de análisis (COA)
Lot/Batch Number
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Protocolos
Storage - Preparation for Culturing - Culturing HEK - Subculturing HEK
Storage - Preparation for Culturing - Culturing HEK - Subculturing HEK
Storage - Preparation for Culturing - Culturing HEK - Subculturing HEK
Storage - Preparation for Culturing - Culturing HEK - Subculturing HEK
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Are there any modifications applied to the 102-05A cell line from primary cells? I am conducting experiments with this cell line, but the cells are growing much faster than I expected.
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